公开(公告)号：US20230174909A1

公开(公告)日：2023-06-08

申请号：US17997991

申请日：2021-05-06

Applicant: MCMASTER UNIVERSITY

Inventor： Boyang ZHANG ,  Shravanthi RAJASEKAR ,  Dawn song YI LIN

IPC: C12M1/32 ,  C12M1/00 ,  C12M1/12

CPC classification number: C12M23/12 ,  C12M29/10 ,  C12M25/14

Abstract: Described herein is a cell culture system for constructing a perfusable network of self-assembled cells comprising a multi-well plate embedded with microchannels connecting a central well with at least one inlet well and at least one outlet well, the central well for culturing seeded cells within an extracellular matrix, wherein the perfusable network allows perfusion through the microchannels connecting the central well with at least one inlet well and at least one outlet well. The cell culture system allows the array of perfusable networks formed, connected, and perfused inside the multi-well plate to be accessible and/or extractable from the top of the central well. In aspect, the cell culture system can improve the experimental throughputs of organ-on-a-chip systems and expand the application of microphysiological systems to regenerative cell therapy. A perfusable network of self-assembled cells and method of making thereof using the cell culture system described herein are also provided.