公开(公告)号：US20230174941A1

公开(公告)日：2023-06-08

申请号：US17921346

申请日：2021-01-14

申请人： MEDICAL CORPORATION YANAGA CLINIC

发明人： Hiroko YANAGA ,  Katsu YANAGA

IPC分类号： C12N5/077 ,  C12M1/42 ,  C12M1/12

CPC分类号： C12N5/0653 ,  C12M35/04 ,  C12M37/02 ,  C12N2501/115 ,  C12N2501/39 ,  C12N2501/2308 ,  C12N2501/21 ,  C12N2501/2306

摘要： A method for producing a mature adipocyte-containing composition includes: a cleavage-filtration step for cleaving and filtering fatty tissue that is not treated with an enzyme and obtaining a minimal fat; a minimal fat culture step for culturing the minimal fat, after the cleavage-filtration step, by means of a culture medium, and obtaining mature adipocytes; and a mature adipocyte-containing composition obtaining step for obtaining a mature adipocyte-containing composition including the mature adipocytes and a conditioned medium obtained from the minimal fat culture step, wherein the culture medium contains an autoserum or contains FBS, hydrocortisone, and FGF-2.