公开(公告)号：US20230174941A1

公开(公告)日：2023-06-08

申请号：US17921346

申请日：2021-01-14

申请人： MEDICAL CORPORATION YANAGA CLINIC

发明人： Hiroko YANAGA ,  Katsu YANAGA

IPC分类号： C12N5/077 ,  C12M1/42 ,  C12M1/12

CPC分类号： C12N5/0653 ,  C12M35/04 ,  C12M37/02 ,  C12N2501/115 ,  C12N2501/39 ,  C12N2501/2308 ,  C12N2501/21 ,  C12N2501/2306

摘要： A method for producing a mature adipocyte-containing composition includes: a cleavage-filtration step for cleaving and filtering fatty tissue that is not treated with an enzyme and obtaining a minimal fat; a minimal fat culture step for culturing the minimal fat, after the cleavage-filtration step, by means of a culture medium, and obtaining mature adipocytes; and a mature adipocyte-containing composition obtaining step for obtaining a mature adipocyte-containing composition including the mature adipocytes and a conditioned medium obtained from the minimal fat culture step, wherein the culture medium contains an autoserum or contains FBS, hydrocortisone, and FGF-2.

公开(公告)号：US20180311313A1

公开(公告)日：2018-11-01

申请号：US15938839

申请日：2018-03-28

申请人： TOKUSHIMA UNIVERSITY

发明人： Akihito YAMAMOTO ,  Minoru UEDA ,  Kohki MATSUBARA ,  Akio SUZUMURA ,  Koichi FURUKAWA ,  Yoshihiro MATSUSHITA ,  Hirotaka WAKAYAMA ,  Nobunori TAKAHASHI ,  Shin TSUNEKAWA ,  Takako IZUMOTO

IPC分类号： A61K38/19 ,  C12N5/0786 ,  A61K45/06 ,  A61K38/17 ,  A61K31/737

CPC分类号： A61K38/195 ,  A61K31/737 ,  A61K38/178 ,  A61K45/06 ,  C12N5/0645 ,  C12N2501/21 ,  C12N2501/59 ,  C12N2501/90 ,  A61K2300/00

摘要： A composition having tissue repair activity, which is capable of promoting reactions associated with tissue repair, contains at least one selected from the group consisting of a first component that is a protein having a monocyte chemotactic protein-1 (MCP-1) activity, a second component that is a protein having the extracellular domain activity of sialic acid-binding immunoglobulin-type lectin-9 (Siglec-9), and a third component that is at least one of chondroitin sulfate and chondroitin sulfate proteoglycan.

公开(公告)号：US09803164B2

公开(公告)日：2017-10-31

申请号：US14854480

申请日：2015-09-15

申请人： New York Blood Center, Inc.

发明人： Beau W. Mitchell

IPC分类号： C12M1/00 ,  C12N5/078

CPC分类号： C12M23/58 ,  C12M29/04 ,  C12M47/02 ,  C12N5/0644 ,  C12N2500/02 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/21 ,  C12N2521/00

摘要： Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.

公开(公告)号：US09757430B2

公开(公告)日：2017-09-12

申请号：US14469963

申请日：2014-08-27

申请人： Cook General Biotechnology LLC

发明人： Erik J. Woods ,  Christopher Taylor

IPC分类号： A61K38/21 ,  A61K35/16 ,  A61K38/19 ,  A61K38/20 ,  A61K38/18 ,  A61K35/14 ,  A61K35/19 ,  C12N5/00

CPC分类号： A61K35/19 ,  A61K35/16 ,  A61K38/1709 ,  A61K38/18 ,  A61K38/1808 ,  A61K38/1825 ,  A61K38/1841 ,  A61K38/1858 ,  A61K38/1866 ,  A61K38/191 ,  A61K38/195 ,  A61K38/20 ,  A61K38/2006 ,  A61K38/204 ,  A61K38/2053 ,  A61K38/2066 ,  A61K38/2086 ,  A61K38/217 ,  A61K38/363 ,  A61K38/38 ,  A61K47/02 ,  A61K47/14 ,  A61K47/26 ,  C12N5/0018 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/165 ,  C12N2501/21 ,  C12N2501/998 ,  C12N2502/115

摘要： The present disclosure provides a composition comprising a bioactive fraction derived from a platelet concentrate, methods of making the bioactive fraction, and culture medium supplemented with the bioactive fraction. Preferred bioactive fractions have relatively low fibrinogen concentrations while retaining native growth factors in beneficial amounts and ratios.

公开(公告)号：US20170239299A1

公开(公告)日：2017-08-24

申请号：US15443602

申请日：2017-02-27

申请人： DePuy Synthes Products, Inc.

发明人： Anthony J. Kihm ,  Ian Ross Harris ,  Sanjay Mistry ,  Alexander M. Harmon ,  Darin J. Messina ,  Agnieszka Seyda ,  Chin-Feng Yi ,  Anna Gosiewska

IPC分类号： A61K35/50 ,  C12N5/073

CPC分类号： A61K35/51 ,  A01N1/0226 ,  A61K35/12 ,  A61K35/50 ,  A61K38/18 ,  A61K38/1808 ,  A61K38/1825 ,  A61K38/1833 ,  A61K38/1841 ,  A61K38/185 ,  A61K38/1858 ,  A61K38/1866 ,  A61K38/1891 ,  A61K38/19 ,  A61K38/204 ,  A61K38/2053 ,  A61K38/27 ,  A61K45/06 ,  C12N5/0605 ,  C12N5/0606 ,  C12N5/0607 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/44 ,  C12N2500/90 ,  C12N2500/95 ,  C12N2501/12 ,  C12N2501/21 ,  C12N2501/23 ,  C12N2502/02 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2509/00 ,  C12N2509/10 ,  C12N2533/50

摘要： Cells derived from postpartum placenta and methods for their isolation are provided by the invention. The invention further provides cultures and compositions of the placenta-derived cells. The placenta-derived cells of the invention have a plethora of uses, including but not limited to research, diagnostic, and therapeutic applications.

公开(公告)号：US09717763B2

公开(公告)日：2017-08-01

申请号：US14018842

申请日：2013-09-05

申请人： DePuy Synthes Products, LLC.

发明人： Sanjay Mistry ,  Anthony J. Kihm ,  Ian R. Harris ,  Alexander M. Harmon ,  Darin J. Messina ,  Agnieszka Seyda ,  Chin-Feng Yi ,  Anna Gosiewska

IPC分类号： A61K35/12 ,  A61K35/51 ,  A61K45/06 ,  C12N5/073 ,  C12N5/074

CPC分类号： A61K35/51 ,  A01N1/0226 ,  A61K35/12 ,  A61K35/50 ,  A61K38/18 ,  A61K38/1808 ,  A61K38/1825 ,  A61K38/1833 ,  A61K38/1841 ,  A61K38/185 ,  A61K38/1858 ,  A61K38/1866 ,  A61K38/1891 ,  A61K38/19 ,  A61K38/204 ,  A61K38/2053 ,  A61K38/27 ,  A61K45/06 ,  C12N5/0605 ,  C12N5/0606 ,  C12N5/0607 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/44 ,  C12N2500/90 ,  C12N2500/95 ,  C12N2501/12 ,  C12N2501/21 ,  C12N2501/23 ,  C12N2502/02 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2509/00 ,  C12N2509/10 ,  C12N2533/50

摘要： Cells derived from human umbilical cords are disclosed along with methods for their therapeutic use. Isolation techniques, culture methods and detailed characterization of the cells with respect to their cell surface markers, gene expression, and their secretion of trophic factors are described.

公开(公告)号：US20170121682A1

公开(公告)日：2017-05-04

申请号：US15402044

申请日：2017-01-09

申请人： New York Blood Center, Inc.

发明人： W. Beau Mitchell ,  Mauro P. Avanzi

IPC分类号： C12N5/078 ,  C12M1/00

CPC分类号： C12N5/0644 ,  C12M23/58 ,  C12M47/02 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/21 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2502/1171 ,  C12N2506/02 ,  C12N2506/1307 ,  C12N2506/45 ,  C12N2521/00

摘要： Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.

公开(公告)号：US09603899B2

公开(公告)日：2017-03-28

申请号：US13877272

申请日：2011-10-03

申请人： Jeremy J. Mao ,  Wenli Zhao

发明人： Jeremy J. Mao ,  Wenli Zhao

IPC分类号： A61K38/18 ,  A61F13/00 ,  A61F2/00 ,  C12N5/00 ,  C12N5/0775 ,  A61L27/54 ,  A61K9/00

CPC分类号： A61K38/1858 ,  A61K9/00 ,  A61L27/54 ,  A61L2300/414 ,  C12N5/0068 ,  C12N5/0663 ,  C12N2501/105 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/21 ,  C12N2533/40 ,  C12N2533/54 ,  C12N2533/74

摘要： Provided is a method of causing a cell to migrate to a scaffold. Also provided is a method of treating a mammal that has a tissue defect. Further provided is a tissue scaffold comprising platelet-derived growth factor (PDGF). Additionally, a method of making a tissue scaffold capable of recruiting a cell is provided.

公开(公告)号：US20170044493A1

公开(公告)日：2017-02-16

申请号：US14936729

申请日：2015-11-10

申请人： KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

发明人： Byung Soo Kim ,  Ji-Hye Jung ,  Yong Park

IPC分类号： C12N5/073 ,  C07K14/52

CPC分类号： C12N5/0603 ,  C07K14/522 ,  C07K14/5421 ,  C12N5/0606 ,  C12N2501/21 ,  C12N2501/998 ,  C12N2506/02 ,  C12N2506/45

摘要： The present invention relates to a method of inducing the differentiation of human pluripotent stem cell-derived embryoid bodies into ectoderm by CXCR2 stimulation, and more particularly, to a method of promoting the differentiation of human pluripotent stem cell-derived embryoid bodies into ectoderm by stimulating and activating the surface receptor CXCR2 of the embryoid bodies with the CXCR2-specific ligand GRO-α. The method of inducing the differentiation of human pluripotent stem cell-derived embryoid bodies into ectoderm by CXCR2 stimulation according to the present invention can increase the efficiency and utility of stem cells as a cell therapeutic agent, because it promotes the differentiation of stem cells into a specific germ layer serving as the origin of target cells, which is the first important step for inducing the differentiation of stem cells into specific cells.

摘要翻译： 本发明涉及通过CXCR2刺激诱导人多能干细胞衍生的胚状体分化为外胚层的方法，更具体地，涉及通过刺激促进人多能干细胞衍生的胚状体分化为外胚层的方法 并用CXCR2特异性配体GRO-α激活胚状体的表面受体CXCR2。 通过根据本发明的CXCR2刺激诱导人多能干细胞衍生的胚状体分化为外胚层的方法可以提高作为细胞治疗剂的干细胞的效率和效用，因为它促进干细胞分化为 作为靶细胞起源的特异性胚层，其是诱导干细胞分化为特定细胞的第一重要步骤。

公开(公告)号：US09498501B2

公开(公告)日：2016-11-22

申请号：US12389305

申请日：2009-02-19

申请人： Sanjay Mistry ,  Anthony J. Kihm ,  Ian R. Harris ,  Alexander M. Harmon ,  Darin J. Messina ,  Agnieszka Seyda ,  Chin-Feng Yi ,  Anna Gosiewska

发明人： Sanjay Mistry ,  Anthony J. Kihm ,  Ian R. Harris ,  Alexander M. Harmon ,  Darin J. Messina ,  Agnieszka Seyda ,  Chin-Feng Yi ,  Anna Gosiewska

IPC分类号： C12N5/073 ,  A61K35/51 ,  A61K35/50 ,  C12N5/074 ,  A61K45/06 ,  A61K35/12

CPC分类号： A61K35/51 ,  A01N1/0226 ,  A61K35/12 ,  A61K35/50 ,  A61K38/18 ,  A61K38/1808 ,  A61K38/1825 ,  A61K38/1833 ,  A61K38/1841 ,  A61K38/185 ,  A61K38/1858 ,  A61K38/1866 ,  A61K38/1891 ,  A61K38/19 ,  A61K38/204 ,  A61K38/2053 ,  A61K38/27 ,  A61K45/06 ,  C12N5/0605 ,  C12N5/0606 ,  C12N5/0607 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/44 ,  C12N2500/90 ,  C12N2500/95 ,  C12N2501/12 ,  C12N2501/21 ,  C12N2501/23 ,  C12N2502/02 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2509/00 ,  C12N2509/10 ,  C12N2533/50

摘要： Cells derived from human umbilical cords are disclosed along with methods for their therapeutic use. Isolation techniques, culture methods and detailed characterization of the cells with respect to their cell surface markers, gene expression, and their secretion of trophic factors are described.