公开(公告)号：US20160312231A1

公开(公告)日：2016-10-27

申请号：US14930710

申请日：2015-11-03

Applicant: MERIAL, INC.

Inventor： Jean-Christophe Francis Audonnet ,  Edmond Jolivet

IPC: C12N15/73 ,  C12N15/85 ,  C12P21/00 ,  C12N15/63

Abstract: The present invention provides a method of maintaining a gram negative bacterium plasmid without the use of antibiotic selection pressure. Further, the invention relates to the drugless plasmids produced including drugless plasmids containing a heterologous gene. The invention also provides formulations and/or compositions comprising the drugless plasmids comprising a heterologous gene, formulations and/or compositions comprising a protein or an immunogen expressed using the drugless plasmids, and methods of administering such formulations and/or compositions to a host. The invention relates to gram negative bacteria containing the drugless plasmids.

Abstract translation: 本发明提供了不使用抗生素选择压力来维持革兰氏阴性细菌质粒的方法。 此外，本发明涉及包含含有异源基因的无毒素质粒产生的无毒素质粒。 本发明还提供了包含含有异源基因，包含使用无药物质粒表达的蛋白质或免疫原的制剂和/或组合物的制剂和/或组合物的制剂和/或组合物，以及向宿主施用这些制剂和/或组合物的方法。 本发明涉及含有无毒素质粒的革兰氏阴性细菌。