公开(公告)号：US20200216838A1

公开(公告)日：2020-07-09

申请号：US16624779

申请日：2017-06-20

Applicant: MGI TECH CO., LTD.

Inventor： Lin Yang ,  Guodong Huang ,  Ya Gao ,  Yanyan Zhang ,  Haiping Zhang ,  Fang Chen ,  Haojun Jiang ,  Shaohong Zhang ,  Yuqian Wang ,  Yifan Xie ,  Hui Jiang

IPC: C12N15/10 ,  C12N15/11

Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on end 3′ of the first primer, and the first random sequence is located on end 5′ of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on end 3′ of the second primer, and the second random sequence is located on end 5′ of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence, respectively; the first random sequence and the second random sequence are reverse complementary; a predetermined restriction enzyme cutting site is connected between the first specific sequence and the first random sequence; and a predetermined restriction enzyme cutting site is connected between the second specific sequence and the second random sequence.

公开(公告)号：US11739319B2

公开(公告)日：2023-08-29

申请号：US16624779

申请日：2017-06-20

Applicant: MGI TECH CO., LTD.

Inventor： Lin Yang ,  Guodong Huang ,  Ya Gao ,  Yanyan Zhang ,  Haiping Zhang ,  Fang Chen ,  Haojun Jiang ,  Shaohong Zhang ,  Yuqian Wang ,  Yifan Xie ,  Hui Jiang

IPC: C12Q1/68 ,  C12P19/34 ,  C12N15/10 ,  C12N15/11

CPC classification number: C12N15/1093 ,  C12N15/11

Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on end 3′ of the first primer, and the first random sequence is located on end 5′ of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on end 3′ of the second primer, and the second random sequence is located on end 5′ of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence, respectively; the first random sequence and the second random sequence are reverse complementary; a predetermined restriction enzyme cutting site is connected between the first specific sequence and the first random sequence; and a predetermined restriction enzyme cutting site is connected between the second specific sequence and the second random sequence.