公开(公告)号：US3843453A

公开(公告)日：1974-10-22

申请号：US33740773

申请日：1973-03-02

Applicant: MILES LAB

Inventor： FREAKE R

IPC: C12N1/04 ,  C12Q1/06 ,  C12K1/04

CPC classification number: C12Q1/06 ,  C12N1/04 ,  Y10S435/822 ,  Y10S435/849 ,  Y10S435/852 ,  Y10S435/873 ,  Y10S435/879 ,  Y10S435/883 ,  Y10S435/885

Abstract: 1. A PROCESS FOR DETRRMINING THE CONSISTENCY AND QUALITY OF MICROBIOLOGICAL GROWTH SUPPORTING MEDIA, WHICH COMPRISES CONTACTING SAID MEDIUM WITH A COMPOSITION COMPRISING A PREDETERMINED QUALITY OF A MICROORGANISM CANABLE OF PRODUCING COLONIES IN OR ON SAID MEDIUM, SAID PREDETERMINED QUANTITY BEING AT LEAST THE MINIMUM INOCULUM REQUIRED TO PRODUCE OBSERVABLE COLONY FORMATION UNDER PREDETEMINED CONDITIONS OF INCUBATION, AND A MICROBIOLOGICALLY INERT COLLOIDAL SUSPENDING AGENT, INCUBATING SAID MEDIUM AND SAID COMPOSITION UNDER PREDETERMINED CONDITIONS AND OBSERVING THE NUMBER OF MICROBIOLOGICAL COLONIES THUS PRODUCED.

公开(公告)号：US3843452A

公开(公告)日：1974-10-22

申请号：US24775172

申请日：1972-04-26

Applicant: MILES LAB

Inventor： FREAKE R ,  HADEN B

IPC: C12M1/34 ,  C12M1/12 ,  C12B1/04

CPC classification number: C12M23/04 ,  C12M25/02 ,  C12M25/14

Abstract: 1. IN AN ARTICLE OF MANUFACTURE FOR USE IN MICROBIOLOGY COMPRISING AN ABSORBENT WEB HAVING A SUBSTANTIALLY FLAT SURFACE AND A MICROPOROUS MEMBRANE OVERLAYING AT LEAST A PORTION OF SAID SURFACE WHICH ARTICLE IS FOR USE IN MICROBIOLOGICAL APPLICATIONS WHEREIN A MICROORGANISM IS CONTACTED WITH THE SURFACE OF SAID MICROPOROUS MEMBRANE, THE IMPROVEMENT WHEREIN SAID MEMBRANE HAS A MEANS PORE SIZE GREATER THAN ABOUT 0.01 MICROMETERS WHILE SMALL ENOUGH TO PROVENT PASSAGE OF SAID MICROORGANISM THERETHROUGH AND SAID WEB AND MEMBRANE BEING JOINTED BY A

CONTINUOUS INTERFACE THEREBETWEEN, INTEGRAL THEREWITH, AND COMPRISING A COMPOSITE, OF THE WEB AND MEMBRANE.

公开(公告)号：US3814670A

公开(公告)日：1974-06-04

申请号：US24786872

申请日：1972-04-26

Applicant: MILES LAB

Inventor： FREAKE R ,  HADEN B

IPC: C12M1/34 ,  C12M1/16 ,  C12M1/20 ,  C12B1/00

CPC classification number: C12M23/04 ,  C12M25/14 ,  C12Q1/04 ,  G01N33/52 ,  Y10S435/805

Abstract: TEST ARTICLE FOR USE IN CULTURING, DETECTING AND ANALYZING MICROORGAISM COMPRISING AN ABSORBENT WEB AND A SUBSTANTIALLY DRY, STABLE, CONTINUOUS LAYER OF A GEL SUCH AS AGAR OR GELATIN JOINED IN LAMINAR RELATIONSHIP THEREWITH BY A CONTINUOUS INTERFACE INTERGAL WITH SAID WEB AND GEL LAYER AND COMPRISING A COMPOSITE OF THE WEB AND GEL LAYER SAID DEVICE MAY BE ADDITIONALLY COMPRISED OF ONE OR MORE SUBSTANTIALLY DRY, STABLE, CNTINUOUS OVERLAYERS OF A GEL POSITIONED IN LAMINAR RELATIONSHIP WITH THE INITIAL GEL LAYER. SUCH GEL OVERLAYER IS PREFERABLY INCORPORATED WITH A REAGENT WHICH INCLUDES MICROBIOLOGICAL NUTRIENT MEDIA, BACKGROUND DYES AND COLOR INDICATORS.