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公开(公告)号：US11442066B2
公开(公告)日：2022-09-13
申请号：US16486723
申请日：2018-02-19
申请人： MITSUI CHEMICALS, INC. , NATIONAL INSTITUTES OF BIOMEDICAL INNOVATION, HEALTH AND NUTRITION , SAVID THERAPEUTICS INC.
发明人： Tsuneji Suzuki , Yoshiyuki Totani , Kosuke Mano , Shinichi Banba , Haruhiko Kamada , Taisuke Nakayama , Hiroki Akiba , Kouhei Tsumoto , Tsuyoshi Inoue
IPC分类号： G01N33/58 , G01N33/68 , C07K16/28 , C07K14/36 , C07D495/04 , C07D519/00
摘要： Finding a protein of a minute amount present on a cell membrane to provide a method for producing an antibody against the protein. Producing an antibody using a protein identified by an identification method including: a labeling step of using a labeling agent comprising at least one selected from bis-iminobiotin compounds and bis-biotin compounds to obtain cells having a labeled protein; a degradation step of preparing a degradation product for an immobilization treatment, the degradation product containing the labeled protein; an immobilization step of immobilizing the labeled protein contained in the degradation product for an immobilization treatment on a stationary phase via a streptavidin mutant; a cleavage step of releasing an analysis sample from the stationary phase on which the labeled protein is immobilized; and an analysis step of analyzing the analysis sample to identify the labeled protein.
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公开(公告)号：US11286285B2
公开(公告)日：2022-03-29
申请号：US15119441
申请日：2015-02-18
发明人： Akira Sugiyama , Hirofumi Doi , Tatsuhiko Kodama , Tsuyoshi Inoue , Eiichi Mizohata , Tatsuya Kawato , Tomohiro Meshizuka , Motomu Kanai , Yohei Shimizu , Noriaki Takasu , Mari Takatsu
IPC分类号： A61B5/00 , A61B8/00 , A61B10/00 , C07K14/36 , A61K49/00 , C07D519/00 , C07D495/04 , C07K16/22 , C07K16/28 , C09B23/10 , C07K14/00 , A61K49/10
摘要： An object of this invention is to provide a streptavidin mutant reduced in affinity to the naturally-occurring biotin, and to provide a modified biotin which shows a high affinity to such streptavidin mutant reduced in affinity to the naturally-occurring biotin. This invention can provide a compound composed of a dimer of modified biotin, a streptavidin mutant, and usage of them.
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公开(公告)号：US11124549B2
公开(公告)日：2021-09-21
申请号：US16512894
申请日：2019-07-16
发明人： Akira Sugiyama , Hirofumi Doi , Tatsuhiko Kodama , Tsuyoshi Inoue , Eiichi Mizohata , Tatsuya Kawato , Tomohiro Meshizuka , Motomu Kanai , Yohei Shimizu , Noriaki Takasu , Mari Takatsu
IPC分类号： A61B5/00 , A61B8/00 , A61B10/00 , C07K14/36 , A61K49/00 , C07D519/00 , C07D495/04 , C07K16/22 , C07K16/28 , C09B23/10 , C07K14/00
摘要： An object of this invention is to provide a streptavidin mutant reduced in affinity to the naturally-occurring biotin, and to provide a modified biotin which shows a high affinity to such streptavidin mutant reduced in affinity to the naturally-occurring biotin. This invention can provide a compound composed of a dimer of modified biotin, a streptavidin mutant, angsd usage of them.
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公开(公告)号：US09670255B2
公开(公告)日：2017-06-06
申请号：US14768916
申请日：2014-02-18
发明人： Akira Sugiyama , Hirofumi Doi , Tatsuhiko Kodama , Tsuyoshi Inoue , Eiichi Mizohata , Tatsuya Kawato , Tomohiro Meshizuka , Motomu Kanai , Yohei Shimizu , Tomohiro Yamamoto
IPC分类号： C07K14/36 , C07D495/04 , C07D497/04 , C07D498/04 , G01N33/543
CPC分类号： C07K14/36 , C07D495/04 , C07D497/04 , C07D498/04 , G01N33/54306 , G01N2333/36
摘要： It is an object of the present invention to provide a mutant streptavidin with a reduced affinity for natural biotin, and also to provide a modified biotin having a high affinity for the mutant streptavidin with a reduced affinity for natural biotin. According to the present invention, there is provided a reagent kit for use in treatments or diagnoses, which comprises: (a) a mutant streptavidin with a reduced affinity for natural biotin or biocytin; and a modified biotin having a high affinity for the above-described mutant streptavidin.
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公开(公告)号：US11161884B2
公开(公告)日：2021-11-02
申请号：US16512894
申请日：2019-07-16
发明人： Akira Sugiyama , Hirofumi Doi , Tatsuhiko Kodama , Tsuyoshi Inoue , Eiichi Mizohata , Tatsuya Kawato , Tomohiro Meshizuka , Motomu Kanai , Yohei Shimizu , Noriaki Takasu , Mari Takatsu
IPC分类号： A61B5/00 , A61B8/00 , A61B10/00 , C07K14/36 , A61K49/00 , C07D519/00 , C07D495/04 , C07K16/22 , C07K16/28 , C09B23/10 , C07K14/00
摘要： An object of this invention is to provide a streptavidin mutant reduced in affinity to the naturally-occurring biotin, and to provide a modified biotin which shows a high affinity to such streptavidin mutant reduced in affinity to the naturally-occurring biotin. This invention can provide a compound composed of a dimer of modified biotin, a streptavidin mutant, angsd usage of them.
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