Image sequence and evaluation method and system for structured illumination microscopy
    1.
    发明授权
    Image sequence and evaluation method and system for structured illumination microscopy 有权
    图像序列和结构照明显微镜的评估方法和系统

    公开(公告)号:US09568304B2

    公开(公告)日:2017-02-14

    申请号:US14612370

    申请日:2015-02-03

    CPC classification number: G01B11/0608 G01B11/2513 G01B11/2527 G02B21/364

    Abstract: In a method and apparatus for determining the height of a plurality of spatial positions on a surface of a specimen, a light beam is projected on the surface. The surface is scanned along an optical axis in different scanning positions. The light reflected by the surface is detected in scanning positions with a spatial pattern having corresponding spatial pattern positions. From the detected light for each spatial position of the surface, an envelope curve of intensity values corresponding to scanning positions is determined. A maximum of the envelope curve and its corresponding scanning position being representative of the height of the spatial position of the surface is selected. The spatial pattern is moved in a sequence of 2n steps (n>2) in a first and a second spatial direction over a distance of ¼ and 1/n pattern wavelength, respectively.

    Abstract translation: 在用于确定样本表面上的多个空间位置的高度的方法和装置中,光束被投射在表面上。 沿不同扫描位置的光轴扫描表面。 在具有对应的空间图案位置的空间图案的扫描位置中检测由表面反射的光。 从针对表面的每个空间位置的检测光,确定与扫描位置对应的强度值的包络线。 选择最大包络线及其对应的扫描位置代表表面空间位置的高度。 空间图案分别在1/4和1 / n图案波长的距离上沿第一和第二空间方向以2n个步长(n> 2)的顺序移动。

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