公开(公告)号：US20030143737A1

公开(公告)日：2003-07-31

申请号：US09732520

申请日：2000-12-07

Applicant: MONASH UNIVERSITY

Inventor： John Roderick Morrison ,  Eric Shannon Hayes ,  Martin Frederick Pera ,  Orly Lacham-Kaplan ,  Alan Osborne Trounson

IPC: C12N005/08

CPC classification number: A01K67/0275 ,  A01K2217/05 ,  A61K35/12 ,  C12N5/0623 ,  C12N15/8775 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2510/04

Abstract: The present invention generally relates to neural stem cells, preferably foetal neural stem cells and their progeny thereof. The present invention provides methods of isolating, culturing and propagating neural stem cells preferably foetal neural stem cells and the development of neural stem cell lines and lineages. The present invention also relates to the use of neural stem cells and somatic cells (eg rat fetal fibroblasts) and cells expressing the telomerase catalytic component (TERT) for gene targeting and gene knockout experiments and for producing genetically modified animals. In a first aspect of the present invention there is provided a cellular composition comprising one or more cells having a property characteristic of a neural stem cell and wherein said neural stem cell is capable of long term culture. Preferably the cells have a property characteristic of a foetal neural stem cell. In another aspect of the present invention there is provided a method of producing an animal, said method comprising introducing a continuously growing donor cell nucleus from a continuously growing donor cell into an oocyte or embryo and allowing the resulting embryo to mature and to preferably develop to a foetus or an adult animal.

Abstract translation: 本发明一般涉及神经干细胞，优选胎儿神经干细胞及其后代。 本发明提供了分离，培养和繁殖神经干细胞，优选胎儿神经干细胞和神经干细胞系和谱系发育的方法。 本发明还涉及神经干细胞和体细胞（例如大鼠胎儿成纤维细胞）和表达端粒酶催化组分（TERT）的细胞用于基因靶向和基因敲除实验以及用于生产转基因动物的用途。 在本发明的第一方面，提供了包含一种或多种具有神经干细胞特性特征的细胞的细胞组合物，其中所述神经干细胞能够进行长期培养。 优选地，细胞具有胎儿神经干细胞的特性。 在本发明的另一方面，提供了一种生产动物的方法，所述方法包括将连续生长的供体细胞核从连续生长的供体细胞引入卵母细胞或胚胎中，并使所得胚胎成熟并优选发育成 胎儿或成年动物。