公开(公告)号：US20130212748A1

公开(公告)日：2013-08-15

申请号：US13682774

申请日：2012-11-21

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Judith C. Donovan ,  William P. Donovan ,  James T. Engleman ,  Thomas M. Malvar ,  John W. Pitkin

IPC: C07K14/32 ,  C12N15/82

CPC classification number: C07K14/32 ,  C07K14/325 ,  C12N15/8286

Abstract: The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

Abstract translation: 本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。 从苏云金芽孢杆菌和相关菌株的培养上清液中分离蛋白质，并显示对包括欧洲玉米螟（ECB），烟草芽孢杆菌（TBW）和小菜蛾（DBM）等鳞翅目昆虫的杀虫活性。 公开了在严格条件下与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。 公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。