公开(公告)号：US06864076B2

公开(公告)日：2005-03-08

申请号：US09258031

申请日：1999-02-25

申请人： Maarten Hendrik Stuiver ,  Jerome Hubertus Henricus Victor Custers ,  Marianne Beatrix Sela-Buurlage ,  Leo Sjoerd Melchers ,  Wessel Lageweg ,  Anne Silene Ponstein ,  Johanna Pieternella Els Van Deventer-Troost

发明人： Maarten Hendrik Stuiver ,  Jerome Hubertus Henricus Victor Custers ,  Marianne Beatrix Sela-Buurlage ,  Leo Sjoerd Melchers ,  Wessel Lageweg ,  Anne Silene Ponstein ,  Johanna Pieternella Els Van Deventer-Troost

IPC分类号： A01H5/00 ,  C07K14/415 ,  C07K16/40 ,  C12N5/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N15/09 ,  C12N15/62 ,  C12N15/82 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12N9/0004 ,  A01N65/12 ,  C07K14/415 ,  C12N9/0006 ,  C12N15/8282

摘要： The present invention provides an isolated protein obtainable from a plant source which has antifungal activity, specifically anti-Phytophthora activity and/or anti-Pythium activity and a molecular weight of about 55-65 kDa as judged by SDS PAGE-electrophoresis, an isolated DNA sequence comprising an open reading frame capable of encoding a protein according to the invention, preferably characterized in that it comprises an open reading frame which is capable of encoding a protein depicted in SEQ ID NO. 16, SEQ ID NO. 57, SEQ ID NO. 70, SEQ ID NO. 72 or SEQ ID NO. 74 or muteins thereof, and DNA capable of hybridizing therewith under stringent conditions. The invention further comprises plants incorporating chimeric DNA capable of encoding a protein according to the invention, and wherein the protein is expressed. Also shown is the carbohydrate and preferably hexose oxidating activity of said protein. Also methods are provided for combating fungi, especially Phytophthora and Pythium species, using a protein or a host cell capable of producing the protein.

摘要翻译： 本发明提供从植物来源获得的分离的蛋白质，其具有抗真菌活性，特别是抗疫苗活性和/或抗腐霉菌活性，并且通过SDS PAGE-电泳判断分子量为约55-65kDa，分离的DNA 其包含能够编码本发明的蛋白质的开放阅读框，其特征在于，其包含能够编码SEQ ID NO：1所示蛋白质的开放阅读框。 16，SEQ ID NO： 57，SEQ ID NO： 70，SEQ ID NO： 72或SEQ ID NO。 74或突变蛋白，以及能够在严格条件下与其杂交的DNA。 本发明还包括含有能够编码本发明的蛋白质的嵌合DNA的植物，并且其中所述蛋白质被表达。 还显示了所述蛋白质的碳水化合物和优选己糖氧化活性。 还提供了使用能够产生蛋白质的蛋白质或宿主细胞来对抗真菌，特别是疫霉属（Phytophthora）和腐霉属（Pythium）物种的方法。