公开(公告)号：US20080052785A1

公开(公告)日：2008-02-28

申请号：US11842406

申请日：2007-08-21

申请人： Marc Caron ,  Vania Prado ,  Marco Prado ,  Raul Gainetdinov ,  Grace Pereira ,  Braulio Castro ,  Cristina Silva ,  Ivan Izquierdo

发明人： Marc Caron ,  Vania Prado ,  Marco Prado ,  Raul Gainetdinov ,  Grace Pereira ,  Braulio Castro ,  Cristina Silva ,  Ivan Izquierdo

IPC分类号： A01K67/027 ,  G01N33/00

CPC分类号： C07K14/70571 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2217/20 ,  A01K2227/105 ,  A01K2267/0356 ,  C12N15/8509 ,  C12N2800/30

摘要： Recombinant non-human mammals having reduced or no expression of vesicular acetylcholine transporter protein (VAChT) as compared to the corresponding wild-type mammal are provided. The mammal may have, e.g., impaired performance in object and social recognition and/or impaired neuromuscular performance and/or alterations in autonomic nervous system function as compared to the corresponding wild-type mammal. Methods of screening a compound for cholinergic activity or activity in treating a cholinergic neurotransmission disorder are also provided. In addition, a cell such as a nerve cell isolated from a mammal as described herein is provided, along with cell cultures, which are useful in vitro for screening the activity of candidate compounds for their effect on cholinergic neurotransmission, and for their activity in treating cholinergic neurotransmission disorders.

摘要翻译： 提供了与相应的野生型哺乳动物相比具有减少或不表达水泡乙酰胆碱转运蛋白（VAChT）的重组非人哺乳动物。 与对应的野生型哺乳动物相比，哺乳动物可能具有例如对象和社会认知和/或受损的神经肌肉表现和/或自主神经系统功能的改变的性能受损。 还提供了筛选化合物胆碱能活性或治疗胆碱能神经传递障碍的活性的方法。 此外，提供了如本文所述的从哺乳动物分离的神经细胞的细胞以及细胞培养物，细胞培养物可用于体外筛选候选化合物对胆碱能神经传递的影响的活性，以及​​它们在治疗中的活性 胆碱能神经传递障碍。