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    Biosynthetic Production of 4-Amino 4-Deoxychorismate (Adc) and [3R,4R]-4-Amino-3-Hydroxycyclohexa-1,5-Diene-1-Carboxylic Acid (3,4-Cha)
    1.
    发明申请
    Biosynthetic Production of 4-Amino 4-Deoxychorismate (Adc) and [3R,4R]-4-Amino-3-Hydroxycyclohexa-1,5-Diene-1-Carboxylic Acid (3,4-Cha) 审中-公开
    4-氨基-4-脱氧胸腺嘧啶（Adc）和[3R，4R] -4-氨基-3-羟基环己-1,5-二烯-1-羧酸（3,4-Cha）的生物合成生产

    公开(公告)号：US20080311631A1

    公开(公告)日：2008-12-18

    申请号：US11628459

    申请日：2005-06-02

    申请人： Marcel Gerhardus Wubbolts ,  Roelof Ary Lans Bovenberg ,  Georg Sprenger ,  Johannes Josef Bongaerts ,  Stefan Kozak ,  Michael Muller ,  Volker Lorbach

    发明人： Marcel Gerhardus Wubbolts ,  Roelof Ary Lans Bovenberg ,  Georg Sprenger ,  Johannes Josef Bongaerts ,  Stefan Kozak ,  Michael Muller ,  Volker Lorbach

    IPC分类号： C12P13/22 ,  C12P13/00 ,  C12N15/63 ,  C12N1/20 ,  C07C61/22

    CPC分类号： C12P13/04

    摘要： The invention relates to a process for the biosynthetic production of 4-amino-4-deoxychorismate (ADC) performed fermentatively in vivo with a 4-amino-4-deoxychorismate synthase, preferably a PabAB bipartite protein (which may be a fusion protein), at an increased level of activity, thereby obtaining a broth comprising ADC and 4-amino-4-deoxyprephenate (ADP), that are recovered. The invention also relates to a further process of converting the ADP into p-aminophenylalanine. The invention, moreover relates to biosynthetic production of [3R,4R]-4-amino-3-hydroxycyclohexa-1,5-diene-1-carboxylic acid (3,4-CHA), by concerted action of such 4-amino-4-deoxychorismate synthase and of an enzyme capable of converting isochorismate into [5S,6S]-5,6 dihydroxycyclohexa-1,3-diene-1-carboxylic acid (2,3-CHD), preferably a phenazine biosynthesis protein PhzD, including recovery of 3,4-CHA. The invention also relates to expression vectors and host cells for use in any of such processes. The invention further relates to the use of 3,4-CHA as a catalytically active product, in particular as a chiral catalyst. And the invention finally relates to synthesis of oseltamivir phosphate from 3,4-CHA.

    摘要翻译： 本发明涉及用4-氨基-4-脱氧激酶合酶，优选PabAB二分体蛋白（可以是融合蛋白）在体内发酵生物合成生产4-氨基-4-脱氧胸苷酸（ADC）的方法。 在增加的活性水平上，从而获得包含ADC和4-氨基-4-脱氧降钙素（ADP）的肉汤，其被回收。 本发明还涉及将ADP转化为对氨基苯丙氨酸的另一种方法。 本发明还涉及通过这种4-氨基-3-羟基环己基-1,5-二烯-1-羧酸（3,4-CHA）的共同作用生产合成[3R，4R] -4-氨基-3-羟基环己-1,5-二烯-1-羧酸（3,4-CHA） 能够将等分子酸转化为[5S，6S] -5,6二羟基环己-1,3-二烯-1-羧酸（2,3-CHD），优选吩嗪生物合成蛋白PhzD的酶的酶，包括4-脱氧胸苷酸合酶 回收3,4-CHA。 本发明还涉及用于任何这些方法的表达载体和宿主细胞。 本发明还涉及3,4-CHA作为催化活性产物，特别是作为手性催化剂的用途。 本发明最终涉及从3,4-CHA合成磷酸奥司他韦。