公开(公告)号：US08153127B2

公开(公告)日：2012-04-10

申请号：US12278023

申请日：2007-02-07

申请人： Marcelo Paez-Pereda ,  Marta Labeur

发明人： Marcelo Paez-Pereda ,  Marta Labeur

IPC分类号： A61K39/395 ,  A61K39/40 ,  A61K39/42 ,  A01N61/00

CPC分类号： G01N33/5038 ,  G01N33/5041 ,  G01N2333/72 ,  G01N2333/726 ,  G01N2800/301 ,  G01N2800/302 ,  G01N2800/304

摘要： The present invention relates to a method for identifying a TMEFF2 modulator, comprising (a) contacting a cell which expresses TMEFF2 with a candidate compound to be tested; (b) measuring whether said compound to be tested decreases or increases the level of a constituent of the cAMP signalling pathway, preferably the CRH signalling pathway, in said cell when compared to a corresponding cell which does not express TMEFF2; (b′) optionally determining whether said compound is capable of reduncing the binding between Activin and TMEFF2; and (c) identifying said modulator compound. Furthermore, a method for identifying a TMEFF2 modulator comprising determining whether said TMEFF2 modulator is capable of reducing the binding between Activin and TMEFF2 is contemplated. It also relates to uses and methods applying a TMEFF2 agonist for the treatment of Cushing's Syndromes and a TMEFF2 modulator for the treatment of affective disorders. Furthermore, methods of diagnosing affective disorders or Cushing's Syndromes are provided.

摘要翻译： 本发明涉及鉴定TMEFF2调节剂的方法，其包括（a）使表达TMEFF2的细胞与待测试的候选化合物接触; （b）当与不表达TMEFF2的相应细胞相比时，测量所述待测化合物是否降低或增加所述细胞中cAMP信号传导途径（优选CRH信号传导途径）的成分的水平; （b'）任选地确定所述化合物是否能够重新激活激活素和TMEFF2之间的结合; 和（c）鉴定所述调节剂化合物。 此外，考虑了用于鉴定TMEFF2调节剂的方法，其包括确定所述TMEFF2调节剂是否能够降低活化素和TMEFF2之间的结合。 它还涉及应用TMEFF2激动剂治疗库兴综合征和用于治疗情感障碍的TMEFF2调节剂的用途和方法。 此外，提供了诊断情感障碍或库兴氏综合征的方法。

公开(公告)号：US20100069463A1

公开(公告)日：2010-03-18

申请号：US12278023

申请日：2007-02-07

申请人： Marcelo Paez-Pereda ,  Marta Labeur

发明人： Marcelo Paez-Pereda ,  Marta Labeur

IPC分类号： A61K31/7088 ,  G01N33/53 ,  G01N33/566 ,  C12Q1/68

CPC分类号： G01N33/5038 ,  G01N33/5041 ,  G01N2333/72 ,  G01N2333/726 ,  G01N2800/301 ,  G01N2800/302 ,  G01N2800/304

摘要： The present invention relates to a method for identifying a TMEFF2 modulator, comprising (a) contacting a cell which expresses TMEFF2 with a candidate compound to be tested; (b) measuring whether said compound to be tested decreases or increases the level of a constituent of the cAMP signalling pathway, preferably the CRH signalling pathway, in said cell when compared to a corresponding cell which does not express TMEFF2; (b′) optionally determining whether said compound is capable of reduncing the binding between Activin and TMEFF2; and (c) identifying said modulator compound. Furthermore, a method for identifying a TMEFF2 modulator comprising determining whether said TMEFF2 modulator is capable of reducing the binding between Activin and TMEFF2 is contemplated. It also relates to uses and methods applying a TMEFF2 agonist for the treatment of Cushing's Syndromes and a TMEFF2 modulator for the treatment of affective disorders. Furthermore, methods of diagnosing affective disorders or Cushing's Syndromes are provided.

摘要翻译： 本发明涉及鉴定TMEFF2调节剂的方法，其包括（a）使表达TMEFF2的细胞与待测试的候选化合物接触; （b）当与不表达TMEFF2的相应细胞相比时，测量所述待测化合物是否降低或增加所述细胞中cAMP信号传导途径（优选CRH信号传导途径）的成分的水平; （b'）任选地确定所述化合物是否能够重新激活激活素和TMEFF2之间的结合; 和（c）鉴定所述调节剂化合物。 此外，考虑了用于鉴定TMEFF2调节剂的方法，其包括确定所述TMEFF2调节剂是否能够降低活化素和TMEFF2之间的结合。 它还涉及应用TMEFF2激动剂治疗库兴综合征和用于治疗情感障碍的TMEFF2调节剂的用途和方法。 此外，提供了诊断情感障碍或库兴氏综合征的方法。