公开(公告)号：US20080015145A1

公开(公告)日：2008-01-17

申请号：US11484364

申请日：2006-07-11

申请人： Maria Gyongyossy-Issa ,  Dana V. Devine ,  Iren Constantinescu ,  William Campbell ,  Carlos del Carpio Munoz

发明人： Maria Gyongyossy-Issa ,  Dana V. Devine ,  Iren Constantinescu ,  William Campbell ,  Carlos del Carpio Munoz

IPC分类号： A61K38/17 ,  C07K14/705

CPC分类号： C07K14/705 ,  A61K38/00 ,  A61K47/60

摘要： Mimotope receptors and inhibitors employ peptide mimics that mimic the shape and function of natural receptors and ligands, thus providing synthetic binding sites for ligands and receptors. Receptor mimics can be attached to carriers, such as liposomes, to act as synthetic platelets, for example, by providing binding sites for binding to other (natural or synthetic) platelets or to the endothelium. Synthetic platelets would have virtually limitless shelf life and would not require disease screening prior to transfusion, thereby providing a solution to the perpetual platelet shortages, as well as the safety and storage issues associated with natural blood platelets. Mimotope inhibitors (either free-molecule receptors or ligands) can act as antithrombotics by inhibiting platelet-platelet or platelet-endothelium interactions. Ligand mimics are preferably D-peptides that resist proteolytic degradation. Furthermore, these ligand mimics can also be attached to carriers for resisting excretion, thus forming the basis for a new class of antithrombotic drugs.

摘要翻译： 模拟表位受体和抑制剂使用模仿天然受体和配体的形状和功能的肽模拟物，从而为配体和受体提供合成结合位点。 受体模拟物可以连接到载体，例如脂质体，以作为合成血小板，例如通过提供与其他（天然或合成的）血小板或内皮结合的结合位点。 合成血小板将具有几乎无限的保质期，并且在输血之前不需要疾病筛查，从而提供对永久血小板缺乏症以及与天然血小板相关的安全和储存问题的解决方案。 模拟表位抑制剂（自由分子受体或配体）可以通过抑制血小板 - 血小板或血小板 - 内皮相互作用而作为抗血栓形成。 配体模拟物优选是抵抗蛋白水解降解的D肽。 此外，这些配体模拟物也可以连接到载体以抵抗排泄，从而形成新类型抗血栓药物的基础。

公开(公告)号：US20080063621A1

公开(公告)日：2008-03-13

申请号：US11851671

申请日：2007-09-07

申请人： Maria Gyongyossy-Issa ,  Jayachandran Kizhakkedathu ,  Iren Constantinescu

发明人： Maria Gyongyossy-Issa ,  Jayachandran Kizhakkedathu ,  Iren Constantinescu

IPC分类号： A61K31/01 ,  A61K31/74 ,  A61P43/00

CPC分类号： A61K9/1271 ,  A61K9/1277 ,  A61K35/19 ,  A61K47/6911

摘要： A method for producing a composition of lipidic particles coated with a cross-linked surface mesh, the method comprising the steps of: (i) preparing lipidic particles comprising pharmaceutically acceptable lipids, (ii) binding hydrophilic polymer chains to the surface of the lipidic particles, and (iii) cross-linking the hydrophilic polymer chains to form the cross-linked surface mesh. Pharmaceutical compositions comprising surface modified lipidic particles prepared according to this method are also described. The lipidic particles resist fusion with red blood cells and platelets in vitro, and are amenable to further derivatization by targeting molecules for controlled release of component and contents, thus providing a new generation of drug carrier systems.

摘要翻译： 一种制备涂覆有交联表面网的脂质颗粒的组合物的方法，所述方法包括以下步骤：（i）制备包含药学上可接受的脂质的脂质颗粒，（ii）将亲水性聚合物链结合到脂质颗粒的表面 ，和（iii）交联亲水性聚合物链以形成交联表面网。 还描述了包含根据该方法制备的表面改性脂质颗粒的药物组合物。 脂质颗粒体外抵抗与红细胞和血小板的融合，并且适于通过靶向分子进一步衍生化以控制组分和内容物的释放，从而提供新一代的药物载体体系。

公开(公告)号：US20080044803A1

公开(公告)日：2008-02-21

申请号：US11888003

申请日：2007-07-31

申请人： Maria Gyongyossy-Issa ,  Jerry Zhang

发明人： Maria Gyongyossy-Issa ,  Jerry Zhang

IPC分类号： A01N1/02 ,  C12N5/08

CPC分类号： A01N1/02 ,  A01N1/021

摘要： Disclosed is a platelet additive solution (PAS) comprising a viscosity increasing agent. The use of a PAS comprising such a viscosity increasing agent, especially at concentrations effective to achieve a viscosity similar to that of blood plasma, promotes platelet recovery during extraction from pooled buffy coats and provides for easier platelet production by maintaining the red cell/platelet-rich-supernatant interface.

摘要翻译： 公开了包含增粘剂的血小板添加剂溶液（PAS）。 使用包含这种粘度增加剂的PAS，特别是在有效实现与血浆相似的粘度的浓度下促进从合并的血沉棕黄层提取期间的血小板恢复，并通过维持红细胞/血小板衍生物来提供更容易的血小板产生， 富 - 上清液界面。

公开(公告)号：US20060188867A1

公开(公告)日：2006-08-24

申请号：US11340483

申请日：2006-01-27

申请人： Jason Acker ,  Maria Gyongyossy-Issa

发明人： Jason Acker ,  Maria Gyongyossy-Issa

IPC分类号： A01N1/02 ,  C12N5/08

CPC分类号： A01N1/02 ,  A01N1/0221

摘要： A method for cryopreserving cells entails the liposomal delivery of intracellular sugar(s), such as trehalose, sucrose, raffinose, stachyose, and combinations thereof, into cells and tissues, such as red blood cells, for enhancing post-thaw viability. This method enables rapid and easy delivery of protective molecules into cells which thus greatly simplifies the preparation of cells for cryopreservation. Furthermore, as much lower concentrations of intracellular protectant are used, the method allows red blood cells containing the liposomally-delivered intracellular sugar to be transfused into a patient immediately following the thaw without having to first remove any of the cryoprotectant sugar.

摘要翻译： 用于冷冻保存细胞的方法需要将细胞内糖（例如海藻糖，蔗糖，棉子糖，水苏糖及其组合）的脂质体递送到细胞和组织例如红细胞中，以增强解冻后的活力。 该方法能够将保护性分子快速且容易地递送到细胞中，从而大大简化了用于冷冻保存的细胞的制备。 此外，使用更低浓度的细胞内保护剂，该方法允许含有脂质体递送的细胞内糖的红细胞在解冻后立即输入患者，而不必首先除去任何冷冻保护剂糖。