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    Method of cryopreserving cells and tissues by liposomal delivery of sugars to enhance post-thaw viability
    1.
    发明申请
    Method of cryopreserving cells and tissues by liposomal delivery of sugars to enhance post-thaw viability 审中-公开
    通过脂质体递送糖来冷冻细胞和组织以增强解冻后活力的方法

    公开(公告)号：US20060188867A1

    公开(公告)日：2006-08-24

    申请号：US11340483

    申请日：2006-01-27

    申请人： Jason Acker ,  Maria Gyongyossy-Issa

    发明人： Jason Acker ,  Maria Gyongyossy-Issa

    IPC分类号： A01N1/02 ,  C12N5/08

    CPC分类号： A01N1/02 ,  A01N1/0221

    摘要： A method for cryopreserving cells entails the liposomal delivery of intracellular sugar(s), such as trehalose, sucrose, raffinose, stachyose, and combinations thereof, into cells and tissues, such as red blood cells, for enhancing post-thaw viability. This method enables rapid and easy delivery of protective molecules into cells which thus greatly simplifies the preparation of cells for cryopreservation. Furthermore, as much lower concentrations of intracellular protectant are used, the method allows red blood cells containing the liposomally-delivered intracellular sugar to be transfused into a patient immediately following the thaw without having to first remove any of the cryoprotectant sugar.

    摘要翻译： 用于冷冻保存细胞的方法需要将细胞内糖（例如海藻糖，蔗糖，棉子糖，水苏糖及其组合）的脂质体递送到细胞和组织例如红细胞中，以增强解冻后的活力。 该方法能够将保护性分子快速且容易地递送到细胞中，从而大大简化了用于冷冻保存的细胞的制备。 此外，使用更低浓度的细胞内保护剂，该方法允许含有脂质体递送的细胞内糖的红细胞在解冻后立即输入患者，而不必首先除去任何冷冻保护剂糖。

    Systems and methods for cell preservation
    2.
    发明申请
    Systems and methods for cell preservation 审中-公开
    细胞保存系统和方法

    公开(公告)号：US20050277107A1

    公开(公告)日：2005-12-15

    申请号：US11035419

    申请日：2005-01-13

    申请人： Mehmet Toner ,  Jason Acker ,  Tani Chen ,  Alex Fowler ,  John Baust ,  Sankha Bhowmick

    发明人： Mehmet Toner ,  Jason Acker ,  Tani Chen ,  Alex Fowler ,  John Baust ,  Sankha Bhowmick

    IPC分类号： A01N1/02

    CPC分类号： A01N1/02 ,  A01N1/0221

    摘要： The present invention generally relates to devices and methods for the preservation of cells using drying, freezing, and other related techniques. In one set of embodiments, the invention allows for the preservation of cells in a dried state. In another set of embodiments, the invention allows for the preservation of cells within a glass or other non-viscous, non-frozen media. In some embodiments, the invention allows for the preservation of cells at temperatures below the freezing point of water, and in some cases at cryogenic temperatures, without inducing ice formation. The cells, in certain embodiments, may be preserved in the presence of intracellular and/or extracellular carbohydrates (which may be the same or different), for example, trehalose and sucrose. Carbohydrates may be transported intracellularly by any suitable technique, for example, using microinjection, or through non-microinjected methods such as through pore-forming proteins, electroporation, heat shock, etc. In certain instances, the glass transition temperature of the cells may be raised, e.g., by transporting a carbohydrate intracellularly. In some cases, the cells may be dried and/or stored, for example, in a substantially moisture-saturated environment or a desiccating environment. The cells may also be stored in a vacuum or a partial vacuum. The cells may be protected from oxygen, moisture, and/or light during storage. In certain cases, an inhibitor, such as a cell death inhibitor, a protease inhibitor, an apoptosis inhibitor, and/or an oxidative stress inhibitor may be used during preservation of the cells. The cells may be stored for any length of time, then recovered to a viable state, e.g., through rehydration, for further use.

    摘要翻译： 本发明一般涉及使用干燥，冷冻和其它相关技术来保存细胞的装置和方法。 在一组实施方案中，本发明允许在干燥状态下保存细胞。 在另一组实施方案中，本发明允许保存玻璃或其它非粘性非冷冻介质中的细胞。 在一些实施方案中，本发明允许在低于水的冰点的温度下保存细胞，并且在一些情况下允许在低温下保存细胞，而不引起冰的形成。 在某些实施方案中，细胞可以在细胞内和/或细胞外碳水化合物（其可以相同或不同）存在下保存，例如海藻糖和蔗糖。 碳水化合物可以通过任何合适的技术，例如使用显微注射，或通过非显微注射方法，例如通过成孔蛋白，电穿孔，热休克等，在细胞内运输。在某些情况下，细胞的玻璃化转变温度可以是 例如，通过在细胞内运输碳水化合物而提高。 在一些情况下，细胞可以干燥和/或储存，例如在基本上湿度饱和的环境或干燥的环境中。 细胞也可以在真空或部分真空中储存。 在储存期间，可以保护细胞免受氧气，水分和/或光的影响。 在某些情况下，可以在细胞保存期间使用抑制剂，例如细胞死亡抑制剂，蛋白酶抑制剂，凋亡抑制剂和/或氧化应激抑制剂。 细胞可以储存任何长度的时间，然后恢复到可行状态，例如通过再水化，以供进一步使用。