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公开(公告)号:US06924117B2
公开(公告)日:2005-08-02
申请号:US09908419
申请日:2001-07-18
申请人: Mario N. Lioubin , Lori Friedman , Marcia Belvin , Jeffery S. Larson , Changyou Chen , Stephanie A. Robertson , Wen Shi , Jocelyn Chan , Danxi Li
发明人: Mario N. Lioubin , Lori Friedman , Marcia Belvin , Jeffery S. Larson , Changyou Chen , Stephanie A. Robertson , Wen Shi , Jocelyn Chan , Danxi Li
IPC分类号: C12N15/09 , A61K38/00 , A61K39/395 , A61K45/00 , A61K48/00 , A61P35/00 , C07K14/47 , C07K14/705 , C12N1/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12P21/02 , C12Q1/68 , A01N37/18
CPC分类号: C07K14/705 , A61K38/00
摘要: Rhomboid Related Proteins (RRPs), involved in the EGFR signaling pathway, are provided. Transgenic, nonhuman mammals containing a transgene encoding an RRP polypeptide or a gene effecting the expression of an RRP polypeptide, along with methods of modulating the interaction of RRP proteins with their pathway members, and methods of screening for agents that modulate the interaction of an RRP polypeptide with an RRP binding target, such as RRP-specific antibodies and small molecules identified in high throughput screens, are also provided. Modulating agents identified using the methods of the invention can be used to specifically inhibit growth of tumor cells that overexpress an RRP protein.
摘要翻译: 提供参与EGFR信号通路的菱形相关蛋白(RRPs)。 含有编码RRP多肽的转基因或影响RRP多肽表达的基因的转基因非人类哺乳动物以及调节RRP蛋白与其通路成员的相互作用的方法以及筛选调节RRP相互作用的试剂的方法 还提供了具有RRP结合靶的多肽,例如RRP特异性抗体和在高通量筛选中鉴定的小分子。 使用本发明方法鉴定的调节剂可用于特异性抑制过表达RRP蛋白的肿瘤细胞的生长。