公开(公告)号：US6008436A

公开(公告)日：1999-12-28

申请号：US654025

申请日：1996-05-23

申请人： Mark A. Conkling ,  Charles H. Opperman ,  Gregoria N. Acedo ,  Wen Song

发明人： Mark A. Conkling ,  Charles H. Opperman ,  Gregoria N. Acedo ,  Wen Song

IPC分类号： A01H5/02 ,  C07K14/415 ,  C12N15/82 ,  C12N15/00 ,  C12N15/29 ,  A01H5/00

CPC分类号： C07K14/415 ,  A01H5/02 ,  C12N15/8285

摘要： Nematode-resistant transgenic plants are disclosed. The plants comprise plant cells containing a DNA construct comprising a transcription cassette, which construct comprises, in the 5' to 3' direction, a promoter operable in the plant cells, and a DNA comprising at least a portion of a DNA sequence encoding a nematode-inducible transmembrane pore protein in either the sense or antisense orientation. Intermediates for producing the same along with methods of making and using the same are also disclosed. In an alternate embodiment of the invention, the sense or antisense DNA is replaced with a DNA encoding an enzymatic RNA molecule directed against the mRNA transcript of a DNA sequence encoding a nematode-inducible transmembrane pore protein.

摘要翻译： 披露了抗线虫的转基因植物。 植物包含含有包含转录盒的DNA构建体的植物细胞，该构建体在5'至3'方向上包含在植物细胞中可操作的启动子，以及包含编码线虫的DNA序列的至少一部分的DNA 有意义或反义方向的诱导性跨膜孔蛋白。 还公开了用于制备该介质的中间体及其制备和使用方法。 在本发明的替代实施方案中，有义或反义DNA被编码针对编码线虫诱导型跨膜孔蛋白的DNA序列的mRNA转录物的酶RNA分子的DNA替代。