公开(公告)号：US20060263836A1

公开(公告)日：2006-11-23

申请号：US11360072

申请日：2006-02-23

申请人： Mark Connelly ,  Mark Kopnitsky

发明人： Mark Connelly ,  Mark Kopnitsky

IPC分类号： G01N33/555

CPC分类号： G01N33/54313 ,  Y10T436/10

摘要： A method and composition for the purpose of detecting and measuring analytes, such as antibodies, which are capable of binding with certain binding partners such as antigens. A homogenous assay is performed in the presence of free unbound antibodies. Such a homogeneous assay testing for specific antibodies is herein possible by defining of test subsets of microparticles having specific antigens thereon which are capable of binding with specific target antibodies. The microparticle suspension also includes at least two calibration subsets of microparticles having a binding partner thereon with at least two known levels of concentration which is capable of binding with human antibodies for the purpose of assay calibration. A verification subset of microparticles is included with another binding partner thereon at a known concentration, capable of binding with anti-human antibodies. This suspension is incubated with a human sample and then is incubated with a tagging component which is measurable for calibration and verification and finally for determining the concentration of target antibodies bound to the test subsets. A separate verification subset of microparticles is included for verifying the calibration and for yielding a correction factor therefore. A complete calibration calculation is performed for each sample being tested. Preferably, the attaching component of the tagging component is the same molecule as the first binding partner material positioned on the calibration subsets of microparticles.

摘要翻译： 用于检测和测量能够与某些结合配偶体如抗原结合的分析物（例如抗体）的方法和组合物。 在游离未结合的抗体存在下进行均一的测定。 通过在其上具有能够与特异性靶抗体结合的具有特异性抗原的微粒的测试子集来定义对特异性抗体的这种均一测定测试。 微粒悬浮液还包括具有至少两个已知浓度浓度的至少两个具有结合配偶体的微粒的校准子集，其能够与人抗体结合以进行测定校准。 微粒的验证子集与另一个结合配偶体以已知的浓度包含在其中，能够与抗人抗体结合。 将该悬浮液与人样品一起孵育，然后与标记组分一起孵育，标记组分可测量用于校准和验证，最后用于测定与测试子集结合的靶抗体的浓度。 包括微粒的单独验证子集，用于验证校准和因此产生校正因子。 对每个被测样品进行完整的校准计算。 优选地，标记组分的附着成分与位于微粒校准子集上的第一结合配对物质相同。

公开(公告)号：US20120040850A1

公开(公告)日：2012-02-16

申请号：US13062692

申请日：2009-09-09

申请人： Mark Kopnitsky

发明人： Mark Kopnitsky

IPC分类号： C40B30/04

CPC分类号： G01N33/564 ,  G01N33/54313 ,  G01N33/6854

摘要： The present invention relates to methods and compositions for use in the diagnosis, assessment and treatment of patients with suspected autoimmune disorders. In particular, the present invention provides an auto-antibody assessment system that combines a multiplex bead array using purified antigens with human cells. When analysis is performed with the system using means such as flow cytometry, this mixture can provide a more comprehensive auto-antigen profile for assessing disease states in autoimmune disorders.

摘要翻译： 本发明涉及用于诊断，评估和治疗疑似自身免疫性疾病的患者的方法和组合物。 特别地，本发明提供了使用纯化抗原与人细胞组合多重珠粒阵列的自身抗体评估系统。 当使用系统使用诸如流式细胞术的方法进行分析时，该混合物可以提供更全面的自身抗原谱以评估自身免疫疾病中的疾病状态。

公开(公告)号：US07674632B1

公开(公告)日：2010-03-09

申请号：US10295118

申请日：2002-11-15

申请人： Mark Carle Connelly ,  Mark Kopnitsky

发明人： Mark Carle Connelly ,  Mark Kopnitsky

IPC分类号： G01N33/553 ,  G01N33/53

CPC分类号： G01N33/54313 ,  Y10T436/10

摘要： A method and composition for detecting and measuring analytes, such as antibodies, which are capable of binding with certain binding partners such as antigens. A homogenous assay is performed in the presence of free unbound antibodies. Such a homogeneous assay testing for specific antibodies is herein possible by defining of test subsets of microparticles having specific antigens thereon which are capable of binding with specific target antibodies. The microparticle suspension also includes at least two calibration subsets of microparticles having a binding partner thereon with at least two known levels of concentration which is capable of binding with human antibodies for the purpose of assay calibration. A verification subset of microparticles is included with another binding partner thereon at a known concentration, capable of binding with anti-human antibodies. This suspension is incubated with a human sample and then is incubated with a tagging component.

摘要翻译： 用于检测和测量分析物（例如抗体）的方法和组合物，其能够与某些结合配偶体例如抗原结合。 在游离未结合的抗体存在下进行均一的测定。 通过在其上具有能够与特异性靶抗体结合的具有特异性抗原的微粒的测试子集来定义对特异性抗体的这种均一测定测试。 微粒悬浮液还包括具有至少两个已知浓度浓度的至少两个具有结合配偶体的微粒的校准子集，其能够与人抗体结合以进行测定校准。 微粒的验证子集与另一个结合配偶体以已知的浓度包含在其中，能够与抗人抗体结合。 将该悬浮液与人样品一起孵育，然后与标记组分一起温育。