公开(公告)号：US07863006B2

公开(公告)日：2011-01-04

申请号：US11711020

申请日：2007-02-27

申请人： Martin Vey ,  Wiegang Lang ,  Albrecht Groener ,  Anne Bellon

发明人： Martin Vey ,  Wiegang Lang ,  Albrecht Groener ,  Anne Bellon

IPC分类号： G01N33/53 ,  G01N33/542 ,  G01N1/30 ,  G01N33/48 ,  C12P21/08 ,  C07K16/00

CPC分类号： G01N33/6896 ,  C07K16/18 ,  G01N2800/2828

摘要： This application includes description of antibodies for specifically detecting prions of human origin and methods for detecting pathogenic prions. In some embodiments, the antibodies bind to an epitope characteristic of a human prion protein which is not found in the prion proteins of other species. In some embodiments, the antibodies are not cross-reactive with cow, Syrian Gold hamster, mouse, or rat prions. The application also includes a conformation-dependent immunoassay method for detecting pathogenic prions in a sample containing a prion (PrP) protein. The PrP protein may be present in a first conformation and a second conformation, such as PrPc and PrPSc in which the two conformations have different binding affinity for the antibody used to detect them.

摘要翻译： 本申请包括用于特异性检测人源性朊病毒的抗体的描述和用于检测病原性朊病毒的方法。 在一些实施方案中，抗体结合人朊蛋白的表位特征，其在其他物种的朊病毒蛋白中未发现。 在一些实施方案中，抗体不与牛，叙利亚金仓鼠，小鼠或大鼠朊病毒交叉反应。 该应用还包括用于检测含有朊病毒（PrP）蛋白质的样品中病原性朊病毒的构象依赖性免疫测定方法。 PrP蛋白可以以第一构象和第二构象存在，例如PrPc和PrPSc，其中两种构象对于用于检测它们的抗体具有不同的结合亲和力。