摘要:
Complex multidimensional datasets generated by digital imaging spectroscopy can be organized and analyzed by applying software and computer-based methods comprising sorting algorithms. Combinations of these algorithms to images and graphical data, allow pixels or features to be rapidly and efficiently classified into meaningful groups according to defined criteria. Multiple rounds of pixel or feature selection may be performed based on independent sorting criteria. In one embodiment sorting by spectral criteria (e.g., intensity at a given wavelength) is combined with sorting by temporal criteria (e.g., absorbance at a given time) to identify microcolonies of recombinant organisms harboring mutated genes encoding enzymes having desirable kinetic attributes and substrate specificity. Restriction of the set of pixels analyzed in a subsequent sort based on criteria applied in an earlier sort (“sort and lock” analyses) minimize computational and storage resources. User-defined criteria can also be incorporated into the sorting process by means of a graphical user interface that comprises a visualization tools including a contour plot, a sorting bar and a grouping bar, an image window, and a plot window that allow run-time interactive identification of pixels or features meeting one or more criteria, and display of their associated spectral or kinetic data. These methods are useful for extracting information from imaging data in applications ranging from biology and medicine to remote sensing.
摘要:
Complex multidimensional datasets generated by digital imaging spectroscopy can be organized and analyzed by applying software and computer-based methods comprising sorting algorithms. Combinations of these algorithms to images and graphical data, allow pixels or features to be rapidly and efficiently classified into meaningful groups according to defined criteria. Multiple rounds of pixel or feature selection may be performed based on independent sorting criteria. In one embodiment sorting by spectral criteria (e.g., intensity at a given wavelength) is combined with sorting by temporal criteria (e.g., absorbance at a given time) to identify microcolonies of recombinant organisms harboring mutated genes encoding enzymes having desirable kinetic attributes and substrate specificity. Restriction of the set of pixels analyzed in a subsequent sort based on criteria applied in an earlier sort (“sort and lock” analyses) minimize computational and storage resources. User-defined criteria can also be incorporated into the sorting process by means of a graphical user interface that comprises a visualization tools including a contour plot, a sorting bar and a grouping bar, an image window, and a plot window that allow run-time interactive identification of pixels or features meeting one or more criteria, and display of their associated spectral or kinetic data. These methods are useful for extracting information from imaging data in applications ranging from biology and medicine to remote sensing.
摘要:
An adjustable fire pit grill device that includes an elongated central post having a first end secured within the ground in a fire pit, and a second end that extends out of the fire pit such that the central post is in a vertical orientation. The central post includes one or more channels that extend along the length of the central post. A cooking grate is secured within each of the elongated channels, and the grates are shaped as triangles or sections of a circle such that the grates form a circle, rectangle, square, or other shape when arranged around the central post. Each cooking grate can be independently raised and lowered by means of a removable hand crank that turns a spool that holds a length of cable. In this way, users can simultaneously cook various foods at different temperatures by adjusting the height of each grate.
摘要:
An adjustable fire pit grill device that includes an elongated central post having a first end secured within the ground in a fire pit, and a second end that extends out of the fire pit such that the central post is in a vertical orientation. The central post includes one or more channels that extend along the length of the central post. A cooking grate is secured within each of the elongated channels, and the grates are shaped as triangles or sections of a circle such that the grates form a circle, rectangle, square, or other shape when arranged around the central post. Each cooking grate can be independently raised and lowered by means of a removable hand crank that turns a spool that holds a length of cable. In this way, users can simultaneously cook various foods at different temperatures by adjusting the height of each grate.