公开(公告)号：US20110288783A1

公开(公告)日：2011-11-24

申请号：US13150834

申请日：2011-06-01

申请人： Masaaki Oka ,  Yoshihiko Hamamoto ,  Shouichi Hazama ,  Yusuke Fujita ,  Ryouichi Tsunedomi

发明人： Masaaki Oka ,  Yoshihiko Hamamoto ,  Shouichi Hazama ,  Yusuke Fujita ,  Ryouichi Tsunedomi

IPC分类号： G06F19/10

CPC分类号： G06Q50/22 ,  G16B20/00

摘要： A drug effect-adverse effect prediction system includes a clinical data analysis table generating part, for each combination of genotypes relating to a drug effect or adverse effect, for generation of an analysis table for handling cases related to presence or absence of the drug effect or adverse effect. The system also includes a reliability analysis part, a discrimination formula generating part, a prediction part, and a discrimination formula optimizing part.

摘要翻译： 药物效应 - 不利影响预测系统包括临床数据分析表生成部分，对于与药物作用或不良作用有关的基因型的每种组合，用于产生用于处理与药物作用的存在或不存在有关的病例的分析表，或 负面影响。 该系统还包括可靠性分析部分，鉴别公式生成部分，预测部分和鉴别公式优化部分。

公开(公告)号：US20080319711A1

公开(公告)日：2008-12-25

申请号：US12156094

申请日：2008-05-29

申请人： Yoshihiko Hamamoto ,  Masaaki Oka

发明人： Yoshihiko Hamamoto ,  Masaaki Oka

IPC分类号： G06F17/18

CPC分类号： G06F19/24

摘要： To provide an effective factor extraction system in which samples are generated artificially from a sample group and a virtual sample set is created so that even when the number of sample is relatively small, specific factors can be selected/extracted with high reliability by using the virtual sample set. The effective factor extraction system comprises a section (3) for extracting samples arbitrarily from sample sets (X(15), Y(16)) and creating a plurality of paired virtual sample sets (X(17), Y(18)), a statistical quantity operating section (5) for reading out the feature value held by sample contained in respective virtual sample sets and operating the average value and the variance, a section (6) for operating Fisher ratio from the average value (21) and the variance (22), and a section (8) for testing significant factors using the operated Fisher ratio.

摘要翻译： 为了提供一种有效因子提取系统，其中从样本组人工生成样本，并且创建虚拟样本组，使得即使样本数量相对较小，也可以通过使用虚拟样本集来高可靠性来选择/提取特定因子 样本集。 有效因子提取系统包括用于从样本集（X（15），Y（16））任意提取样本并创建多个配对虚拟样本组（X（17），Y（18））的部分（3） 统计量操作部分（5），用于读出包含在各个虚拟样本组中的样本所保持的特征值并操作平均值和方差;从平均值（21）和 方差（22）和用于使用操作的费雪比检验重要因素的部分（8）。

公开(公告)号：US20120065892A1

公开(公告)日：2012-03-15

申请号：US12759263

申请日：2010-04-13

申请人： Masaaki Oka ,  Yoshihiko Hamamoto ,  Hisafumi Okabe

发明人： Masaaki Oka ,  Yoshihiko Hamamoto ,  Hisafumi Okabe

IPC分类号： G06F19/00 ,  G01N33/574 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/10

CPC分类号： G06F19/18 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6886 ,  C12Q2600/118 ,  G01N33/574 ,  G06F19/20

摘要： The present invention relates to a scoring system for the prediction of cancer recurrence by selecting genes and/or proteins whose expression patterns associated with recurrence of cancer, and generating formulae with the selected genes and/or proteins for the prediction of cancer recurrence. The present invention relates to a kit for determining the likelihood of recurrence of cancer, comprising DNA chip, oligonucleotide chip, protein chip, peptides, antibodies, probes and primers that are necessary for effecting DNA microarrays, oligonucleotide microarrays, protein arrays, northern blotting, in situ hybridization, RNase protection assays, western blotting, ELISA assays, reverse transcription polymerase-chain reaction to examine the expression of at least 2, 4, 6, 10, 12 or more genes and/or proteins, that are indicative of cancer recurrence.

摘要翻译： 本发明涉及一种用于通过选择与癌症复发相关的表达模式的基因和/或蛋白质预测癌症复发的评分系统，并且与所选择的基因和/或蛋白质产生用于预测癌症复发的公式。 本发明涉及用于确定癌症复发可能性的试剂盒，其包括DNA芯片，寡核苷酸芯片，蛋白芯片，肽，抗体，探针和引物，其用于实现DNA微阵列，寡核苷酸芯片，蛋白质阵列，Northern印迹， 原位杂交，RNA酶保护测定，蛋白质印迹，ELISA测定，逆转录聚合酶链反应以检测至少2,4,6,10,12或更多个表达癌症复发的基因和/或蛋白质的表达 。

公开(公告)号：US08655597B2

公开(公告)日：2014-02-18

申请号：US12759263

申请日：2010-04-13

申请人： Masaaki Oka ,  Yoshihiko Hamamoto ,  Hisafumi Okabe

发明人： Masaaki Oka ,  Yoshihiko Hamamoto ,  Hisafumi Okabe

IPC分类号： G06F7/00

CPC分类号： G06F19/18 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6886 ,  C12Q2600/118 ,  G01N33/574 ,  G06F19/20

摘要： The present invention relates to a scoring system for the prediction of cancer recurrence by selecting genes and/or proteins whose expression patterns associated with recurrence of cancer, and generating formulae with the selected genes and/or proteins for the prediction of cancer recurrence. The present invention relates to a kit for determining the likelihood of recurrence of cancer, comprising DNA chip, oligonucleotide chip, protein chip, peptides, antibodies, probes and primers that are necessary for effecting DNA microarrays, oligonucleotide microarrays, protein arrays, northern blotting, in situ hybridization, RNase protection assays, western blotting, ELISA assays, reverse transcription polymerase-chain reaction to examine the expression of at least 2, 4, 6, 10, 12 or more genes and/or proteins, that are indicative of cancer recurrence.

摘要翻译： 本发明涉及一种用于通过选择与癌症复发相关的表达模式的基因和/或蛋白质预测癌症复发的评分系统，并且与所选择的基因和/或蛋白质产生用于预测癌症复发的公式。 本发明涉及用于确定癌症复发可能性的试剂盒，其包括DNA芯片，寡核苷酸芯片，蛋白芯片，肽，抗体，探针和引物，其用于实现DNA微阵列，寡核苷酸芯片，蛋白质阵列，Northern印迹， 原位杂交，RNA酶保护测定，蛋白质印迹，ELISA测定，逆转录聚合酶链反应以检测至少2,4,6,10,12或更多个表达癌症复发的基因和/或蛋白质的表达 。

公开(公告)号：US20070292423A1

公开(公告)日：2007-12-20

申请号：US10552178

申请日：2003-04-08

申请人： Masaaki Oka ,  Yoshihiko Hamamoto ,  Norio Iizuka ,  Hisafumi Okabe ,  Kenji Hamada

发明人： Masaaki Oka ,  Yoshihiko Hamamoto ,  Norio Iizuka ,  Hisafumi Okabe ,  Kenji Hamada

IPC分类号： A61K39/395 ,  A61P43/00 ,  C12Q1/68

CPC分类号： G01N33/57484 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/136

摘要： The present invention relates to a method of defining the differentiation grade of tumor by selecting genes and/or proteins whose expression level correlates with each differentiation grade of tumor, measuring the expression of the genes and/or proteins of human tumor tissues in each differentiation grade. The present invention also relates to the use of these genes and/or proteins for diagnosing the differentiation grade of tumor and for screening anti-cancer agents for tumor treatment.

摘要翻译： 本发明涉及通过选择其表达水平与肿瘤的每个分化等级相关的基因和/或蛋白质来测定肿瘤分化等级的方法，测量每个分化等级中人肿瘤组织的基因和/或蛋白质的表达 。 本发明还涉及这些基因和/或蛋白质用于诊断肿瘤分化等级和筛选用于肿瘤治疗的抗癌剂的用途。

公开(公告)号：US08140456B2

公开(公告)日：2012-03-20

申请号：US12156094

申请日：2008-05-29

申请人： Yoshihiko Hamamoto ,  Masaaki Oka

发明人： Yoshihiko Hamamoto ,  Masaaki Oka

IPC分类号： G06F17/15

CPC分类号： G06F19/24

摘要： To provide an effective factor extraction system in which samples are generated artificially from a sample group and a virtual sample set is created so that even when the number of sample is relatively small, specific factors can be selected/extracted with high reliability by using the virtual sample set. The effective factor extraction system comprises a section for extracting samples arbitrarily from sample sets and creating a plurality of paired virtual sample sets, a statistical quantity operating section for reading out the feature value held by sample contained in respective virtual sample sets and operating the average value and the variance, a section for operating Fisher ratio from the average value and the variance, and a section for testing significant factors using the operated Fisher ratio.

摘要翻译： 为了提供一种有效因子提取系统，其中从样本组人工生成样本，并且创建虚拟样本组，使得即使样本数量相对较小，也可以通过使用虚拟样本集来高可靠性来选择/提取特定因子 样本集。 有效因子提取系统包括从样本组任意提取样本并创建多个配对虚拟样本集的部分，统计量操作部分，用于读出包含在各个虚拟样本组中的样本所保存的特征值，并操作平均值 和方差，从平均值和方差操作Fisher比率的部分，以及使用操作的Fisher比率测试显着因子的部分。

公开(公告)号：US08927209B2

公开(公告)日：2015-01-06

申请号：US12528982

申请日：2008-03-01

申请人： Yoshihiko Hamamoto ,  Norio Iizuka ,  Toshiaki Miura ,  Toyoki Moribe ,  Masaaki Oka ,  Shigeru Tamatsukuri

发明人： Yoshihiko Hamamoto ,  Norio Iizuka ,  Toshiaki Miura ,  Toyoki Moribe ,  Masaaki Oka ,  Shigeru Tamatsukuri

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6886 ,  C12Q2523/125 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/156

摘要： The present invention provides a novel method for detection of liver cancer. This method detects high-sensitively, high-specifically, simply and accurately liver cancer, especially that in early stage by identifying and/or quantifying methylation on particular genes and/or their DNA fragments in clinical specimens, and by combining said methylated DNA values with existing tumor marker values and/or DNA amounts in blood. This invention also detects a precancerous lesion, detects a risk of recurrence after treatment of liver cancer, detects malignancy of liver cancer and monitors progression of liver cancer with time by the same method. As for particular genes, BASP1 gene, SPINT2 gene, APC gene, CCND2 gene, CFTR gene, RASSF1 gene and SRD5A2 gene are mentioned.

摘要翻译： 本发明提供一种检测肝癌的新方法。 该方法通过鉴定和/或定量临床标本中特定基因和/或其DNA片段的甲基化，并将所述甲基化DNA值与 血液中存在的肿瘤标志物值和/或DNA量。 本发明还检测癌前病变，检测肝癌治疗后复发的风险，通过相同的方法随时间检测肝癌的恶性程度并监测肝癌的进展。 对于特定基因，提及BASP1基因，SPINT2基因，APC基因，CCND2基因，CFTR基因，RASSF1基因和SRD5A2基因。

公开(公告)号：US20100304372A1

公开(公告)日：2010-12-02

申请号：US12528982

申请日：2008-03-01

申请人： Yoshihiko Hamamoto ,  Norio Iizuka ,  Toshiaki Miura ,  Toyoki Moribe ,  Masaaki Oka ,  Shigeru Tamatsukuri

发明人： Yoshihiko Hamamoto ,  Norio Iizuka ,  Toshiaki Miura ,  Toyoki Moribe ,  Masaaki Oka ,  Shigeru Tamatsukuri

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2523/125 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/156

摘要： The present invention provides a novel method for detection of liver cancer. This method detects high-sensitively, high-specifically, simply and accurately liver cancer, especially that in early stage by identifying and/or quantifying methylation on particular genes and/or their DNA fragments in clinical specimens, and by combining said methylated DNA values with existing tumor marker values and/or DNA amounts in blood. This invention also detects a precancerous lesion, detects a risk of recurrence after treatment of liver cancer, detects malignancy of liver cancer and monitors progression of liver cancer with time by the same method. As for particular genes, BASP1 gene, SPINT2 gene, APC gene, CCND2 gene, CFTR gene, RASSF1 gene and SRD5A2 gene are mentioned.

摘要翻译： 本发明提供一种检测肝癌的新方法。 该方法通过鉴定和/或定量临床标本中特定基因和/或其DNA片段的甲基化，并将所述甲基化DNA值与 血液中存在的肿瘤标志物值和/或DNA量。 本发明还检测癌前病变，检测肝癌治疗后复发的风险，通过相同的方法随时间检测肝癌的恶性程度并监测肝癌的进展。 对于特定基因，提及BASP1基因，SPINT2基因，APC基因，CCND2基因，CFTR基因，RASSF1基因和SRD5A2基因。

公开(公告)号：US07747389B2

公开(公告)日：2010-06-29

申请号：US10484664

申请日：2001-07-23

申请人： Masaaki Oka ,  Yoshihiko Hamamoto ,  Hisafumi Okabe

发明人： Masaaki Oka ,  Yoshihiko Hamamoto ,  Hisafumi Okabe

IPC分类号： G06F7/00

CPC分类号： G06F19/18 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6886 ,  C12Q2600/118 ,  G01N33/574 ,  G06F19/20

摘要： The present invention relates to a scoring system for the prediction of cancer recurrence. More particularly, the present invention concerns with the selection of genes and/or proteins, and generation of formulae with the selected genes and/or proteins for the prediction of cancer recurrence by measuring the expression of genes and/or proteins of human tumor tissues, and comparing their patterns with those of the gene and/or protein expression of human primary tumors from patients who have cancer recurrence and those who do not have cancer recurrence. The present invention also relates to a kit for performing the method of the present invention comprising DNA chip, oligonucleotide chip, protein chip, peptides, antibodies, probes and primers that are necessary for effecting DNA microarrays, oligonucleotide microarrays, protein arrays, northern blotting, in situ hybridization, RNase protection assays, western blotting, ELISA assays, reverse transcription polymerase-chain reaction (hereinafter referred to as RT-PCR) to examine the expression of at least 2 or more of genes and/or proteins, preferably 4 or more of genes and/or proteins, more preferably 6 or more of genes and/or proteins, and most preferably 12 or more of genes and/or proteins, that are indicative of cancer recurrence.

摘要翻译： 本发明涉及一种预测癌症复发的评分系统。 更具体地，本发明涉及基因和/或蛋白质的选择，以及通过测量人肿瘤组织的基因和/或蛋白质的表达，通过选择的基因和/或蛋白质产生具有预测癌症复发的配方， 并将其模式与患有癌症复发的患者的人原发性肿瘤的基因和/或蛋白质表达和不具有癌症复发的患者的模式进行比较。 本发明还涉及用于实施本发明的方法的试剂盒，其包含DNA芯片，寡核苷酸芯片，蛋白芯片，肽，抗体，探针和引物，其用于实现DNA微阵列，寡核苷酸微阵列，蛋白质阵列，Northern印迹， 原位杂交，RNA酶保护测定，蛋白质印迹，ELISA测定，逆转录聚合酶链反应（以下称为RT-PCR）检测至少2个或更多个基因和/或蛋白质的表达，优选4个或更多个 的基因和/或蛋白质，更优选6个或更多个基因和/或蛋白质，最优选12个或更多个表示癌症复发的基因和/或蛋白质。