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公开(公告)号:US20050221320A1
公开(公告)日:2005-10-06
申请号:US10508236
申请日:2003-03-20
CPC分类号: G01N33/5091 , G01N33/6893 , G01N2800/2828
摘要: The present invention relates to a method for detecting a prion in a sample comprising providing a population of cells which are susceptible to prion infection, contacting the population of cells with the cells with the sample, incubating the cells on a growth substrate, assaying for prion colony formation wherein presence of prion colonies indicates presence of prions in the sample. The invention further relates to methods for quantifying the prions, and to cell lines sensitive to prion infection.
摘要翻译: 本发明涉及一种检测样品中朊病毒的方法,包括提供易受朊病毒感染影响的细胞群,将细胞群与细胞接触样品,将细胞孵育在生长底物上,测定朊病毒 集落形成,其中存在朊病毒集落表明样品中存在朊病毒。 本发明还涉及用于量化朊病毒的方法和对朊病毒感染敏感的细胞系。