公开(公告)号：US06333448B1

公开(公告)日：2001-12-25

申请号：US09161994

申请日：1998-09-29

申请人： Maureen Bafor ,  Antoni Banas ,  Anders Dahlqvist ,  Per-Olov Gummeson ,  Michael Lee ,  Staffan Sjodal ,  Sten Stymne ,  Marit Lenman

发明人： Maureen Bafor ,  Antoni Banas ,  Anders Dahlqvist ,  Per-Olov Gummeson ,  Michael Lee ,  Staffan Sjodal ,  Sten Stymne ,  Marit Lenman

IPC分类号： A01H500

CPC分类号： C12N9/0071 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472

摘要： The present invention relates to a novel plant enzyme called delta 12 fatty acid acetylenase. This enzyme is responsible for the conversion of fatty acids to acetylenic acids and the invention relates to production of such acids. The invention also relates to use of cDNA encoding acetylenase, preferably Crepis alpina delta 12 acetylenase, for transforming organisms such as oil accumulating organisms selected from the group consisting of oil crops, oleogeneous yeasts and moulds. Furthermore, the invention relates to organisms such as oil accumulating organisms transformed with acetylenase cDNA, and to oils and other acetylenic compounds from said organisms.

摘要翻译： 本发明涉及称为δ12脂肪酸炔酰酶的新型植物酶。 该酶负责将脂肪酸转化为炔属酸，本发明涉及这种酸的生产。 本发明还涉及使用编码炔酰酶（最好是Crepis alpina delta 12炔基酶）的cDNA转化生物体，例如选自油料作物，不饱和酵母和霉菌的积聚生物体。 此外，本发明涉及生物体，例如用炔基酶cDNA转化的油积聚生物，以及来自所述生物体的油和其它炔属化合物。