公开(公告)号：US20230181466A1

公开(公告)日：2023-06-15

申请号：US17759768

申请日：2021-01-29

申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

发明人： Oskar Staufer ,  Yilia Plazman ,  Joachim P. Spatz

IPC分类号： A61K9/127 ,  C12N15/88 ,  C12N15/113 ,  C07K14/705 ,  A61P17/02 ,  A61K38/45 ,  A61K38/17

CPC分类号： A61K9/1272 ,  C12N15/88 ,  A61K9/1277 ,  C12N15/113 ,  C07K14/70596 ,  C07K14/70503 ,  A61P17/02 ,  A61K38/45 ,  A61K38/1709 ,  A61K38/1761 ,  C07K14/70575 ,  C12Y204/02012 ,  C12N2310/141

摘要： The present invention relates to a method for producing synthetic extracellular vesicles comprising a lipid bilayer including at least two lipids, one or more extracellular vesicle associated proteins, and optionally one or more nucleic acid molecules. The inventive synthetic extracellular vesicles are formed by emulsification using a mechanic emulsifier in the form of polymer shell stabilized synthetic extracellular vesicles. The inventive method allows producing synthetic extracellular vesicles miming the composition and function of natural extracellular vesicles. Therefore, synthetic extracellular vesicles with specific protein and nucleic acids compositions are also disclosed herein, as well as their therapeutic uses.

公开(公告)号：US20200170949A1

公开(公告)日：2020-06-04

申请号：US16623363

申请日：2018-06-06

申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften E.V.

发明人： Joachim P. Spatz ,  Lucia T. Benk ,  Johannes Patrick Frohnmayer ,  Barbara Haller ,  Jan-Willi Janiesch ,  Yilia Plazman ,  Marian Weiss

IPC分类号： A61K9/127

摘要： The present invention relates to a method for preparing a protocell in form of a giant unilamellar vesicle, which comprises the following steps: a) providing a water-based droplet encapsulated by an outer polymer shell, which borders the inner space of the droplet, wherein the droplet has a maximum dimension of 0.5 μm to 1,000 μm, wherein the inner space of the droplet contains at least one lipid, b) transforming the lipid content of the droplet into a lipid bilayer which is arranged at and covers the inner surface of the polymer shell and oil phase in order to form a polymer shell-stabilized giant unilamellar vesicle, c) optionally incorporating one or more proteins and/or nuclei into the polymer shell-stabilized giant unilamellar vesicle provided in step b) and d) optionally removing the polymer shell and oil phase from the polymer shell-stabilized giant unilamellar vesicle and optionally transferring it from the oil to the water phase.