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公开(公告)号：US10781456B2
公开(公告)日：2020-09-22
申请号：US15737558
申请日：2016-06-22
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Yeda Research and Development Co. Ltd., at the Weizmann Institute of Science
IPC分类号： C12N15/82
摘要： The present invention relates to an organism, a tissue, a cell or an organelle expressing enzymes which allow the conversion of 2-phosphoglycolate (2-PG; also known as glycolate 2-phosphate,) into an intermediate compound of the Calvin-Benson-Bassham Cycle (CBBC) without releasing CO2. The organism, tissue, cell or organelle of the invention may be genetically engineered, transgenic and/or transplastomic so as to express at least one enzyme which is involved in this conversion. The present invention further relates to an organism, tissue, cell or organelle which comprises/expresses at least one enzyme which is involved in this conversion. The present invention further relates to a method for producing an organism, tissue, cell or organelle of the invention. The present invention further relates to a method of enzymatically converting 2-PG into an intermediate compound of the CBBC without releasing CO2. The present invention further relates to the use of an organism, tissue, cell or organelle of the invention for enzymatically converting 2-PG into an intermediate compound of the CBBC without releasing CO2.
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公开(公告)号：US11976310B2
公开(公告)日：2024-05-07
申请号：US17800484
申请日：2021-02-16
发明人： Arren Bar-Even , Hai He , Philippe Marliere
CPC分类号： C12P13/04 , C12N15/52 , C12N15/70 , C12Y101/01103 , C12Y101/03013 , C12Y203/01029 , C12Y401/02 , C12Y403/01 , C12N2800/101
摘要： The present disclosure relates to a method for the incorporation of formaldehyde into biomass comprising the following enzymatically catalyzed steps: (1) condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB); (2) amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine; (3) conversion of thus produced homoserine to threonine; (4) conversion of the thus produced threonine into glycine and acetaldehyde or acetyl-CoA; (5) condensation of the thus produced glycine with formaldehyde to produce serine; and (6) conversion of the thus produced serine to produce pyruvate, wherein said pyruvate can then be used as a substrate in step (1). The disclosure also relates to enzymes for catalyzing the corresponding enzymatic reactions and recombinant microorganisms which express the enzymes for catalyzing the corresponding enzymatic reactions.
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