公开(公告)号：US20250011413A1

公开(公告)日：2025-01-09

申请号：US18706314

申请日：2022-11-03

Applicant: McMaster University

Inventor： Ishac Nazy ,  John Kelton ,  Donald Arnold ,  Anna-Lise Bissola ,  Colin Kretz

IPC: C07K16/24 ,  A61K39/00 ,  A61P7/02 ,  G01N33/564 ,  G01N33/68

Abstract: Provided herein are wildtype and mutant single chain variable fragments (scFvs) of the anti-PF4/heparin monoclonal antibody KKO, and variants thereof which are useful for distinguishing between platelet-activating (pathogenic) and non-activating (non-pathogenic) anti-PF4/heparin antibodies. Also provided herein are uses of the scFvs in methods for identifying patients with pathogenic anti-PF4/heparin antibodies, and for diagnosing and treating heparin-induced thrombocytopenia (HIT).

公开(公告)号：US20230406899A1

公开(公告)日：2023-12-21

申请号：US18211970

申请日：2023-06-20

Applicant: McMaster University

Inventor： Ishac Nazy ,  Donald Arnold ,  John Kelton ,  Angela Huynh ,  Mercy Charity Daka

IPC: C07K14/52 ,  G01N33/68 ,  G01N33/564

CPC classification number: C07K14/522 ,  G01N33/6863 ,  G01N2333/522 ,  G01N2800/226 ,  G01N33/564

Abstract: Described are mutant Platelet Factor 4 (PF4) proteins that exhibit different binding affinities to vaccine induced immune thrombotic thrombocytopenia (VITT) antibodies or non-heparin-induced thrombocytopenia (non-HIT) antibodies relative to heparin-induced thrombocytopenia (HIT) antibodies. Also provided herein are methods for differentiating between VITT, HIT, and/or non-HIT in subjects suspected of having VITT, HIT, or non-HIT.