公开(公告)号：US11577248B2

公开(公告)日：2023-02-14

申请号：US16229487

申请日：2018-12-21

Applicant: McMaster University

Inventor： Maikel Rheinstadter ,  Sebastian Himbert ,  Richard Alsop ,  Jose Moran-Mirabal ,  Sokunthearath Saem ,  Dawn Bowdish

IPC: B01L3/00 ,  G01N33/53 ,  G01N27/327 ,  G01N33/49

Abstract: A biosensor detector device is disclosed suitable for use in measuring membrane fluidity or membrane permeability. The biosensor detector device is formed of a solid substrate having a lipid bilayer compatible surface, a multi-lamellar lipid membrane structure derived from a biological cell and localized on the lipid bilayer compatible surface, an aqueous layer interposed between each lipid bilayer of the multi-lamellar lipid membrane structure. The biological membrane is derived from human red blood cells and localized on the lipid bilayer compatible surface. An electrode forming all or part of the lipid bilayer compatible surface may be used to detect disruptions in the multi-lamellar lipid membrane structure and hemolytic activity in a test sample.

公开(公告)号：US20190195867A1

公开(公告)日：2019-06-27

申请号：US16229487

申请日：2018-12-21

Applicant: McMaster University

Inventor： Maikel Rheinstadter ,  Sebastian Himbert ,  Richard Alsop ,  Jose Moran-Mirabal ,  Sokunthearath Saem ,  Dawn Bowdish

IPC: G01N33/543 ,  G01N27/327 ,  G01N33/53

CPC classification number: G01N33/543 ,  G01N27/3272 ,  G01N33/5306

Abstract: A biosensor detector device is disclosed suitable for use in measuring membrane fluidity or membrane permeability. The biosensor detector device is formed of a solid substrate having a lipid bilayer compatible surface, a multi-lamellar lipid membrane structure derived from a biological cell and localized on the lipid bilayer compatible surface, an aqueous layer interposed between each lipid bilayer of the multi-lamellar lipid membrane structure. The biological membrane is derived from human red blood cells and localized on the lipid bilayer compatible surface. An electrode forming all or part of the lipid bilayer compatible surface may be used to detect disruptions in the multi-lamellar lipid membrane structure and hemolytic activity in a test sample.