公开(公告)号：US20190209618A1

公开(公告)日：2019-07-11

申请号：US16096429

申请日：2017-04-26

Applicant: McMaster University

Inventor： Kristin Hope ,  Stefan Rentas

IPC: A61K35/28 ,  A61K31/7048 ,  A61K31/37 ,  A61K31/09 ,  A61P35/02 ,  A61K31/352 ,  C07D311/18 ,  C07D311/32 ,  C07D473/00 ,  C12N15/113 ,  A61P37/00 ,  A61P7/00

CPC classification number: A61K35/28 ,  A61K31/09 ,  A61K31/352 ,  A61K31/37 ,  A61K31/7048 ,  A61K45/06 ,  A61P7/00 ,  A61P35/02 ,  A61P37/00 ,  C07D311/18 ,  C07D311/32 ,  C07D473/00 ,  C12N5/0647 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2501/70 ,  C12N2501/71 ,  C12Y114/14001 ,  A61K2300/00

Abstract: A method of increasing the self-renewal and/or expansion of hematopoietic stem and/or progenitor cells (HSPCs) is described. Inhibiting the activity and/or expression of cytochrome P450 1B1 (CYP1B1) and/or increasing the expression or activity of Musashi-2 (MSI2) increases the expansion of HSPCs. The HSPCs may be cultured in the presence of a CYP1B1 inhibitor and/or a MSI2 activator. Optionally, the cells may be expanded ex vivo and transplanted into a subject in need thereof.