公开(公告)号：US20220065806A1

公开(公告)日：2022-03-03

申请号：US17466354

申请日：2021-09-03

Applicant: McMaster University

Inventor： Leyla Soleymani ,  Sudip Saha ,  Amanda Victorious

IPC: G01N27/30 ,  G01N27/327

Abstract: This disclosure relates to a biosensor for detecting a target analyte in a sample comprising a first and second photoelectrode each comprising conductive substrate and photoactive material, a first and second capture probe functionalized on the first and second photoelectrode, respectively, and optionally one or more reporter moieties comprising a detectable label, wherein the first and second capture probe each, independently, provides a distance between the detectable label and the photoactive material in the presence of the target analyte, wherein intensity of detection signal dictated by the distance is generated from the first and second photoelectrode by transfer of electrons between the detectable label and the photoactive material, wherein a higher, or higher increase than in absence of sample, in the intensity of the detection signal from the first as compared to the second photoelectrode in the presence of the sample, is indicative of the presence of the target analyte.

公开(公告)号：US11874247B2

公开(公告)日：2024-01-16

申请号：US17466354

申请日：2021-09-03

Applicant: McMaster University

Inventor： Leyla Soleymani ,  Sudip Saha ,  Amanda Victorious

IPC: G01N27/30 ,  G01N27/327

CPC classification number: G01N27/305 ,  G01N27/3276

Abstract: Provided herein is a biosensor for detecting a target analyte in a sample comprising a first and second photoelectrode each comprising conductive substrate and photoactive material, a first and second capture probe functionalized on the first and second photoelectrode, respectively, and optionally one or more reporter moieties comprising a detectable label, wherein the first and second capture probe each, independently, provides a distance between the detectable label and the photoactive material in the presence of the target analyte, wherein intensity of detection signal dictated by the distance is generated from the first and second photoelectrode by transfer of electrons between the detectable label and the photoactive material, wherein a higher, or higher increase than in absence of sample, in the intensity of the detection signal from the first as compared to the second photoelectrode in the presence of the sample, is indicative of the presence of the target analyte.

公开(公告)号：US20220221418A1

公开(公告)日：2022-07-14

申请号：US17572376

申请日：2022-01-10

Applicant: McMaster University

Inventor： Leyla Soleymani ,  Sudip Saha ,  Amanda Victorious

IPC: G01N27/30 ,  G01N27/12 ,  G01N27/327 ,  G01N27/02 ,  H01G9/20

Abstract: This disclosure relates to a biosensor, and methods of use thereof, for detecting a target in a sample comprising a photoelectrode comprising a conductive substrate and a photoactive material; a population of capture probes functionalized on the photoelectrode wherein the capture probes are capable of binding to the target and a reporter moiety; and the reporter moiety comprising a detectable label and a capture probe binding portion; wherein exposure of the target to the population of the capture probes results in binding of the target to a fraction of the population which results in a decrease in detection signal intensity compared to the intensity in the absence of the target, and subsequent binding of the reporter moiety to the remaining unbound capture probes results in an increase in detection signal intensity that is less than an increase from the reporter moiety binding to capture probes not exposed to the target.