公开(公告)号：US20210123910A1

公开(公告)日：2021-04-29

申请号：US16999851

申请日：2020-08-21

Applicant: McMaster University

Inventor： Warren G Foster

IPC: G01N33/53 ,  A61K45/06

Abstract: A method of detecting the expression level of miRNA markers in a biological sample obtained from a mammal is provided. The method incudes the steps of i) detecting the expression level of one or more miRNA markers selected from the group of miR-199a-3p, miR-143-3p, miR-340-5p, let-7b-5p, miR-21-5p, miR-17-5p, miR-20a-5p and miR-103a-3p, in the biological sample; ii) detecting the expression level of at least one miRNA reference marker selected from miR-148b-3p and miR-30e-5p in the biological sample; and iii) normalizing the expression level of the miRNA marker(s) against the expression level of the miRNA reference marker in the sample and in a control. The method is useful for the diagnosis of endometriosis, monitoring of patient response to treatment, and assessment of disease progression and/or severity.

公开(公告)号：US20240302358A1

公开(公告)日：2024-09-12

申请号：US18431041

申请日：2024-02-02

Applicant: McMaster University

Inventor： Warren G Foster

IPC: G01N33/53 ,  A61K45/06 ,  C12Q1/6837

CPC classification number: G01N33/5308 ,  A61K45/06 ,  C12Q1/6837 ,  B01J2219/00529 ,  G01N2496/00 ,  G01N2800/364

Abstract: A method of detecting the expression level of miRNA markers in a biological sample obtained from a mammal is provided. The method incudes the steps of i) detecting the expression level of one or more miRNA markers selected from the group of miR-199a-3p, miR-143-3p, miR-340-5p, let-7b-5p, miR-21-5p, miR-17-5p, miR-20a-5p and miR-103a-3p, in the biological sample; ii) detecting the expression level of at least one miRNA reference marker selected from miR-148b-3p and miR-30e-5p in the biological sample; and iii) normalizing the expression level of the miRNA marker(s) against the expression level of the miRNA reference marker in the sample and in a control. The method is useful for the diagnosis of endometriosis, monitoring of patient response to treatment, and assessment of disease progression and/or severity.

公开(公告)号：US11965879B2

公开(公告)日：2024-04-23

申请号：US16999851

申请日：2020-08-21

Applicant: McMaster University

Inventor： Warren G Foster

IPC: A61K45/06 ,  C12Q1/6837 ,  G01N33/53

CPC classification number: G01N33/5308 ,  A61K45/06 ,  C12Q1/6837 ,  B01J2219/00529 ,  G01N2496/00 ,  G01N2800/364

Abstract: A method of detecting the expression level of miRNA markers in a biological sample obtained from a mammal is provided. The method incudes the steps of i) detecting the expression level of one or more miRNA markers selected from the group of miR-199a-3p, miR-143-3p, miR-340-5p, let-7b-5p, miR-21-5p, miR-17-5p, miR-20a-5p and miR-103a-3p, in the biological sample; ii) detecting the expression level of at least one miRNA reference marker selected from miR-148b-3p and miR-30e-5p in the biological sample; and iii) normalizing the expression level of the miRNA marker(s) against the expression level of the miRNA reference marker in the sample and in a control. The method is useful for the diagnosis of endometriosis, monitoring of patient response to treatment, and assessment of disease progression and/or severity.