Purification of oligonucleotides
    1.
    发明授权
    Purification of oligonucleotides 失效
    寡核苷酸的纯化

    公开(公告)号:US08680262B2

    公开(公告)日:2014-03-25

    申请号:US13421273

    申请日:2012-03-15

    IPC分类号: C07H21/00

    CPC分类号: C07H21/00

    摘要: A method for purifying a protected oligonucleotide comprising the steps of: a) providing a solution of the protected oligonucleotide in at least one solvent A having a boiling point below the boiling point of a solvent B, heating the solution at a temperature of at least 30° C. and below the boiling point of the at least solvent A, adding solvent B until precipitation of a material is visible in the solution, said solvent B being an alcohol having 1 to 6 C-atoms or a diol having 2 to 6 C-atoms, allowing the solution to cool down under stirring until formation of a supernatant and a residue, removing the supernatant or b) providing solvent B, said solvent B being an alcohol having 1 to 6 C-atoms or a diol having 2 to 6 C-atoms, heating solvent B at a temperature above 30° C. and below the boiling point of solvent B, adding a solution of a protected oligonucleotide in at least one solvent A until precipitation of a material is visible in the solution, allowing the solution to cool down under stirring until formation of a supernatant and a residue, removing the supernatant.

    摘要翻译: 一种用于纯化受保护的寡核苷酸的方法,包括以下步骤:a)在至少一种沸点低于溶剂B的沸点的溶剂A中提供受保护的寡核苷酸的溶液,在至少30℃的温度下加热该溶液 ℃,并且低于至少溶剂A的沸点,加入溶剂B直到溶液中可观察到材料沉淀,所述溶剂B是具有1至6个C原子的醇或具有2至6个碳原子的二醇 原子,使溶液在搅拌下冷却直到形成上清液和残余物,除去上清液或b)提供溶剂B,所述溶剂B是具有1至6个碳原子的醇或具有2至6个碳原子的二醇 C原子,加热溶剂B,温度高于30℃,低于溶剂B的沸点,在至少一种溶剂A中加入被保护的寡核苷酸的溶液,直到在溶液中析出材料,使得 解决方案冷却下来 在搅拌下直到形成上清液和残余物,除去上清液。

    DEVELOPER UNIT FOR AN ELECTROPHOTOGRAPHIC PRINTING DEVICE FOR PRINTING ON GLASS OR CERAMIC MATERIAL
    2.
    发明申请
    DEVELOPER UNIT FOR AN ELECTROPHOTOGRAPHIC PRINTING DEVICE FOR PRINTING ON GLASS OR CERAMIC MATERIAL 审中-公开
    用于印刷玻璃或陶瓷材料的电子印刷印刷设备的开发单元

    公开(公告)号:US20100196060A1

    公开(公告)日:2010-08-05

    申请号:US12597367

    申请日:2008-04-10

    IPC分类号: G03G15/08

    摘要: The invention relates to a developer unit for an electrophotographic printing device for printing on glass or ceramic material having a toner supply and a magnetic roll (22) for applying toner to a photoconductor (10), which is connected to a substrate to be printed, directly or with the interposition of a transfer device (20). According to the invention, a conditioning medium (24) for transferring the toner acts between the magnetic roll (22) and the photoconductor (10).

    摘要翻译: 本发明涉及一种用于印刷玻璃或陶瓷材料的电子照相印刷装置的显影装置,该装置具有调色剂供应装置和用于将调色剂施加到与待印刷的基板连接的光电导体(10)的磁性辊(22) 直接或通过插入传送设备(20)。 根据本发明,用于转印调色剂的调理介质(24)作用在磁辊(22)和感光体(10)之间。

    DEVELOPER UNIT FOR AN ELECTROPHOTOGRAPHIC PRINTING DEVICE FOR PRINTING ON GLASS OR CERAMIC MATERIAL
    3.
    发明申请
    DEVELOPER UNIT FOR AN ELECTROPHOTOGRAPHIC PRINTING DEVICE FOR PRINTING ON GLASS OR CERAMIC MATERIAL 审中-公开
    用于印刷玻璃或陶瓷材料的电子印刷印刷设备的开发单元

    公开(公告)号:US20100232842A1

    公开(公告)日:2010-09-16

    申请号:US12597350

    申请日:2008-04-10

    IPC分类号: G03G15/08

    CPC分类号: G03G15/0805

    摘要: The invention relates to a developer unit for an electrophotographic printing device for printing on glass or ceramic material having a toner supply (30, 32) and a toner application device (38), toner being discharged onto a developer device (18) by means of the toner application device (38) and it being possible for the developer device (18) to be brought into flat contact with a photoconductor (10). According to the invention, the developer device (18) has a fibre coating (19) of electrically conductive fibres, which picks up the toner and is in contact with the photoconductor (10) in order to transfer the toner.

    摘要翻译: 本发明涉及一种用于在具有调色剂供给(30,32)和调色剂施加装置(38)的玻璃或陶瓷材料上印刷的电子照相印刷装置的显影剂单元,调色剂通过以下方式排放到显影剂装置(18)上: 调色剂施加装置(38),并且显影装置(18)可以与感光体(10)平坦接触。 根据本发明,显影装置(18)具有导电纤维的光纤涂层(19),其拾取调色剂并与感光体(10)接触以转印调色剂。