公开(公告)号：US06767635B1

公开(公告)日：2004-07-27

申请号：US10088437

申请日：2002-05-24

申请人： Michael Bahr ,  Dimitri Berkov ,  Norbert Buske ,  Joachim Clement ,  Peter Görnert ,  Klaus Höffken ,  Kay-Oliver Kliche ,  Thomas Kober ,  Matthias Schnabelrauch ,  Sebastian Vogt ,  Kerstin Wagner ,  Christian Gansau

发明人： Michael Bahr ,  Dimitri Berkov ,  Norbert Buske ,  Joachim Clement ,  Peter Görnert ,  Klaus Höffken ,  Kay-Oliver Kliche ,  Thomas Kober ,  Matthias Schnabelrauch ,  Sebastian Vogt ,  Kerstin Wagner ,  Christian Gansau

IPC分类号： B32B516

CPC分类号： B82Y5/00 ,  A61K47/6923 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2998

摘要： The invention relates to magnetic nanoparticles, their production, and their use. The object of the invention is to provide nanoparticles capable of specifically forming bonds to intracellular biomacromolecules even in the intracellular region of cells, so that separation is possible by exposure to an exterior magnetic field. This is accomplished by means of magnetic nanoparticles having biochemical activity, consisting of a magnetic core particle and an envelope layer fixed to the core particle, and including a compound of general formula M-S-L-Z (I), the linkage sites between S and L and L and Z having covalently bound functional groups, wherein M represents said magnetic core particle; S represents a biocompatible substrate fixed to M; L represents a linker group; and Z represents a group comprised of nucleic acids, peptides or proteins or derivatives thereof, which group has at least one structure capable of specifically binding to a binding domain of an intracellular biomacromolecule.

摘要翻译： 本发明涉及磁性纳米颗粒及其制备及其用途。本发明的目的是提供即使在细胞的细胞内区域也能特异性地形成与细胞内生物大分子的结合的纳米粒子，从而可以通过暴露于外部磁性 这是通过具有生物化学活性的磁性纳米颗粒来实现的，该磁性纳米颗粒由固定在核心颗粒上的磁芯颗粒和包膜层组成，并且包括通式MSLZ（I）的化合物，S和L之间的连接位点和 L和Z具有共价结合的官能团，其中M表示所述磁芯颗粒; S表示固定于M的生物相容性底物; L表示连接基团; Z表示由核酸，肽或蛋白质或其衍生物组成的组，该基团具有至少一种能够特异性结合细胞内生物大分子的结合结构域的结构。