公开(公告)号：US4912208A

公开(公告)日：1990-03-27

申请号：US67833

申请日：1987-06-29

申请人： Michael D. Fiechtner ,  Christopher Bieniarz ,  Mohamed Shipchandler ,  Maciej Adamczyk

发明人： Michael D. Fiechtner ,  Christopher Bieniarz ,  Mohamed Shipchandler ,  Maciej Adamczyk

IPC分类号： G01N33/544 ,  C07D493/10 ,  C07H15/26 ,  G01N33/542 ,  G01N33/543 ,  G01N33/58

CPC分类号： C07D493/10 ,  B82Y5/00 ,  C07H15/26 ,  G01N33/5432 ,  G01N33/586

摘要： The present invention provides novel fluorescein derivative compounds having fluorescence spectrum and quantum yield characteristics similar to those of fluorescein. The compounds are readily synthesized and purified and are readily soluble in water at self-quenching concentrations. Significantly, due to the presence of polar polyhydroxy group substituents and the absence of metal-chelating groups, these fluorescein derivatives are susceptible to minimal leakage across liposome membranes and have fluorescence characteristics minimally sensitive to the presence of metal ions. Compounds of the invention are thus exceptionally suitable for use in the development of highly storage stable liposome preparations to be employed in immunolytic assays involving human body fluid samples.

公开(公告)号：US4970074A

公开(公告)日：1990-11-13

申请号：US367151

申请日：1989-07-24

申请人： Michael D. Fiechtner ,  Christopher Bieniarz ,  Mohamed Shipchandler ,  Maciej Adamczyk

发明人： Michael D. Fiechtner ,  Christopher Bieniarz ,  Mohamed Shipchandler ,  Maciej Adamczyk

IPC分类号： A61K9/127 ,  C07D493/10 ,  C07H15/26 ,  G01N33/543 ,  G01N33/58

CPC分类号： C07D493/10 ,  A61K9/127 ,  C07H15/26 ,  G01N33/5432 ,  G01N33/586

摘要： The present invention provides novel fluorescein derivative compounds having fluorescence spectrum and quantum yield characteristics similar to those of fluorescein. The compounds are readily synthesized and purified and are readily soluble in water at self-quenching concentrations. Significantly, due to the presence of polar polyhydroxy group substituents and the absence of metal-chelating groups, these fluorescein derivatives are susceptible to minimal leakage across liposome membranes and have fluorescence characteristics minimally sensitive to the presence of metal ions. Compounds of the invention are thus exceptionally suitable for use in the development of highly storage stable liposome preparations to be employed in immunolytic assays involving human body fluid samples.

摘要翻译： 本发明提供具有与荧光素相似的荧光光谱和量子产率特征的新型荧光素衍生物化合物。 化合物容易合成和纯化，并且在自熄浓度下易溶于水。 显着地，由于极性多羟基取代基的存在和不存在金属螯合基团，这些荧光素衍生物易受到脂质体膜上的最小渗漏的影响，并且具有对金属离子存在最不敏感的荧光特性。 因此，本发明的化合物特别适用于开发用于涉及人体液样品的免疫分析测定中的高度储存稳定的脂质体制剂。