公开(公告)号：US20240337654A1

公开(公告)日：2024-10-10

申请号：US18749553

申请日：2024-06-20

Applicant: Purdue Research Foundation

Inventor： Joseph Irudayaraj ,  Wen Ren

IPC: G01N33/543 ,  B01L3/00 ,  G01N33/52 ,  G01N33/569 ,  G01N33/58

CPC classification number: G01N33/54388 ,  B01L3/5023 ,  G01N33/521 ,  G01N33/56916 ,  G01N33/586 ,  G01N33/587

Abstract: The present disclosure relates to a novel method for lateral flow immunoassay (LFIA) by utilizing plasmonic enhancement strategy. More specifically, the present disclosure provides a plasmonic enhanced lateral flow sensor (pLFS) concept by introducing a liposome-based amplification of the colorimetric signals on the lateral flow platform for ultrasensitive detection of pathogens.

公开(公告)号：US12000836B2

公开(公告)日：2024-06-04

申请号：US17126583

申请日：2020-12-18

Applicant: Becton, Dickinson and Company

Inventor： Majid Mehrpouyan ,  Oleg Guryev

IPC: G01N33/58 ,  G01N21/64

CPC classification number: G01N33/586 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/582 ,  G01N2496/00

Abstract: Methods of quantitating extra-cellular vesicle surface markers are provided. Aspects of the methods include comparing: a mean fluorescence intensity of a surface marker of interest (surface marker MFI) of a labeled extra-cellular vesicle (EV) sample with a calibration plot obtained from a liposome calibration composition to quantitate the surface marker on extra-cellular vesicles of the EV sample. Also provided are compositions that find use in practicing embodiments of the invention.

公开(公告)号：US20180272008A1

公开(公告)日：2018-09-27

申请号：US15762260

申请日：2016-09-26

Applicant: University of Notre Dame du Lac

Inventor： Zihni Basar BILGICER ,  Peter DEAK ,  Tanyel KIZILTEPE BILGICER ,  Jared Francis STEFANICK ,  Jonathan Darryl ASHLEY

IPC: A61K49/00 ,  C12Q1/40

CPC classification number: A61K49/0006 ,  A61K9/0014 ,  A61K9/1271 ,  A61K39/35 ,  A61K47/6911 ,  A61K2039/60 ,  C12Q1/40 ,  C12Y302/01052 ,  G01N33/586 ,  G01N33/6854 ,  G01N33/686 ,  G01N2800/24

Abstract: Embodiments of the present disclosure provide a nanoparticle based platform, and nanoallergens for identifying, evaluating and studying allergen mimotopes as multiple copies of a single mimotope or various combinations on the same particle. The nanoparticle is extremely versatile and allows multivalent binding to IgEs specific to a variety of mimotopes, simulating allergen proteins. Nanoparticles can include various molecular ratios of components. For example, the nanoallergens can include about 0.1-40% mimotope-lipid conjugate and about 60-99.9% lipid. The mimotope-lipid conjugate includes a mimotope, a first linker, and lipid molecule. Nanoallergens can be used in in vitro and in vivo applications to identify a specific patient's sensitivity to a set of epitopes and predict a symptomatic clinical response, identify allergen epitopes through blind screening peptide sequences from allergen protein, and in a clinical application similar to a scratch test.

公开(公告)号：US20170328922A1

公开(公告)日：2017-11-16

申请号：US15528166

申请日：2015-03-19

Applicant: UNIVERSITY OF SOUTH AUSTRALIA ,  CURTIN UNIVERSITY OF TECHNOLOGY

Inventor： Doug Brooks ,  Sally Plush ,  Massimiliano Massi

IPC: G01N33/92 ,  C07D471/04 ,  G01N33/50 ,  G01N33/58 ,  C07D257/04 ,  G01N33/574 ,  C07F13/00

CPC classification number: G01N33/92 ,  C07D257/04 ,  C07D401/04 ,  C07D471/04 ,  C07F13/00 ,  G01N33/5005 ,  G01N33/574 ,  G01N33/586 ,  G01N2405/02 ,  G01N2405/06 ,  G01N2405/10

Abstract: The present disclosure relates to methods and products for labelling, binding and/or detection of lipids. Certain embodiments provide a method of labelling a lipid. The method comprises exposing the lipid to a complex comprising a transition metal carbonyl compound, a conjugated bidentate ligand and a tetrazolato compound, and thereby labelling the lipid by binding the complex to the lipid.

公开(公告)号：US20160258962A1

公开(公告)日：2016-09-08

申请号：US15155308

申请日：2016-05-16

Applicant: BIOCOMPATIBLES UK LIMITED

Inventor： Giuseppe BATTAGLIA

IPC: G01N33/58

CPC classification number: G01N33/586 ,  G01N33/5005 ,  G01N33/582

Abstract: The present invention relates to a method of monitoring live cells, comprising the sequential steps of: (i) contacting the cells with self-assembled constructs associated with an imaging agent at t=0; (ii) incubating the cells for a period of time t1; (iii) imaging at least a sample of the live cells by means of the said imaging agent after time t1; wherein in step (i), at least some of the constructs are taken up into the cells; and wherein the constructs comprise an amphiphilic block copolymer having a hydrophilic and hydrophobic block.

公开(公告)号：US20150104878A1

公开(公告)日：2015-04-16

申请号：US14241594

申请日：2012-08-30

Applicant: Neil J. Campbell ,  Keith Edward Moravick ,  Joseph D. Penniman ,  Bruce J. Richardson

Inventor： Neil J. Campbell ,  Keith Edward Moravick ,  Joseph D. Penniman ,  Bruce J. Richardson

IPC: G01N33/53

CPC classification number: G01N33/5304 ,  G01N33/5432 ,  G01N33/558 ,  G01N33/586

Abstract: Methods are disclosed for performing a bioassay, comprising activating capsules containing a signal precursor that is hydrolysable from a latent form in which substantially no signal is generated to a form in which it is able to generate a detectable signal, said activating comprising treating said capsules with heat and with an acid or a base catalysing solution, the combination of said heat and the pH of the catalysing solution being such as to hydrolyse said precursor to the form in which it is able to generate a detectable signal.

Abstract translation: 公开了用于进行生物测定的方法，包括活化胶囊，所述胶囊含有信号前体，所述信号前体可潜在地从其中基本上不产生信号的潜在形式到能够产生可检测信号的形式，所述活化包括用 加热和酸或碱催化溶液，所述加热和催化溶液的pH的组合使得将所述前体水解成能够产生可检测信号的形式。

公开(公告)号：US20140234831A1

公开(公告)日：2014-08-21

申请号：US14347682

申请日：2012-09-28

Applicant: CIRLE, Inc.

Inventor： Richard Awdeh

IPC: C12Q1/10 ,  C12Q1/04 ,  C12Q1/14

CPC classification number: C12Q1/10 ,  C12Q1/04 ,  C12Q1/14 ,  G01N33/56911 ,  G01N33/586

Abstract: Provided herein is a composition for detection of a microbial product in a sample comprising a polydiacetylene (PDA) and a packaging polymer, wherein the sample contacts the PDA and a change of PDA color indicates detection. Also provided herein are methods of making a packaging material comprising a PDA and methods of using the composition for detection of a microbial product in a sample.

Abstract translation: 本文提供了用于检测包含聚二乙炔（PDA）和包装聚合物的样品中的微生物产物的组合物，其中样品接触PDA并且PDA颜色的改变指示检测。 本文还提供制备包含PDA的包装材料的方法以及使用该组合物检测样品中微生物产物的方法。

公开(公告)号：US20130323770A1

公开(公告)日：2013-12-05

申请号：US13985559

申请日：2012-02-14

Applicant: Uma Lakshmipathy ,  Upinder Singh ,  Scott Grecian ,  Rene Quintanilla ,  Kyle Gee ,  Mahendra Rao

Inventor： Uma Lakshmipathy ,  Upinder Singh ,  Scott Grecian ,  Rene Quintanilla ,  Kyle Gee ,  Mahendra Rao

IPC: C12Q1/42

CPC classification number: G01N33/5005 ,  C09B11/24 ,  C09B19/00 ,  C09B21/00 ,  C09B57/00 ,  C09B57/02 ,  C12Q1/42 ,  C12Y301/03001 ,  G01N1/30 ,  G01N33/5073 ,  G01N33/581 ,  G01N33/582 ,  G01N33/586 ,  G01N2001/302 ,  G01N2333/916

Abstract: The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.

Abstract translation: 本发明涉及用于染色活的干细胞的新的底物和方法。 染色可用于在体细胞重编程过程中鉴定诱导的多能干细胞集落。

公开(公告)号：US20130052749A1

公开(公告)日：2013-02-28

申请号：US13221600

申请日：2011-08-30

Applicant: Neil J. Campbell ,  Keith Edward Moravick ,  Joseph D. Penniman ,  Bruce J. Richardson

Inventor： Neil J. Campbell ,  Keith Edward Moravick ,  Joseph D. Penniman ,  Bruce J. Richardson

IPC: G01N21/64

CPC classification number: G01N33/5304 ,  G01N33/5432 ,  G01N33/558 ,  G01N33/586

Abstract: A method of performing a bioassay comprising activating capsules containing a signal precursor that is hydrolysable from a latent form in which substantially no signal is generated to a form in which it is able to generate a detectable signal, said activating comprising treating said capsules with heat and with an acid or a base catalysing solution, the combination of said heat and the pH of the catalysing solution being such as to hydrolyse said precursor to the form in which it is able to generate a detectable signal.

Abstract translation: 一种进行生物测定的方法，包括活化胶囊，所述胶囊含有可从潜在形式水解的信号前体，所述潜在形式基本上不产生任何信号而产生可产生可检测信号的形式，所述活化包括用热处理所述胶囊， 使用酸或碱催化溶液，所述热和催化溶液的pH的组合使得将所述前体水解成能够产生可检测信号的形式。

公开(公告)号：US20110065135A1

公开(公告)日：2011-03-17

申请号：US12842663

申请日：2010-07-23

Applicant: Daisuke Okamura ,  Ryusuke Murayama ,  Naoyoshi Egashira

Inventor： Daisuke Okamura ,  Ryusuke Murayama ,  Naoyoshi Egashira

IPC: G01N33/53

CPC classification number: G01N33/586 ,  G01N33/5432

Abstract: A liposome composition capable of including a chemical substance such as an electrochemiluminescent substance in an internal aqueous phase of the liposome at a higher concentration, and a production method thereof, as well as an analytical method of an analyte that enables an analyte to be analyzed with a high sensitivity using the liposome composition are provided. In a liposome composition containing a liposome, and a chemical substance enclosed in an internal aqueous phase of the liposome, a lipid bilayer composing the liposome has a positive or negative charge, and the chemical substance has a charge opposite to the charge of the lipid bilayer.

Abstract translation: 能够以较高浓度在脂质体的内部水相中包含化学物质如电化学发光物质的脂质体组合物及其制备方法，以及分析物的分析方法，使分析物能够用 提供了使用脂质体组合物的高灵敏度。 在包含脂质体的脂质体组合物和包封在脂质体的内部水相中的化学物质中，构成脂质体的脂质双层具有正或负电荷，并且化学物质具有与脂质双层的电荷相反的电荷 。