-
1.Method of producing transgenic maize using direct transformation of commercially important genotypes 失效
标题翻译: 使用商业上重要的基因型的直接转化生产转基因玉米的方法公开(公告)号:US06403865B1
公开(公告)日:2002-06-11
申请号:US08438666
申请日:1995-05-10
申请人: Michael G. Koziel , Nalini M. Desai , Kelly S. Lewis , Vance C. Kramer , Gregory W. Warren , Stephen V. Evola , Lyle D. Crossland , Martha S. Wright , Ellis J. Merlin , Karen L. Launis , Steven J. Rothstein , Cindy G. Bowman , John L. Dawson , Erik M. Dunder , Gary M. Pace , Janet L. Suttie , Nadine Carozzi , Annick De Framond , James O. Linder , Robert L. Miller , Bruce W. Skillings , Alan W. Mousel , Albert R. Hornbrook , Christopher P. Clucas , Moez Rajabali Meghji , Andreas H. Tanner , Francis E. Cassagne , Gilles Pollini , Terry Ray Colbert , Francis P. Cammack
发明人: Michael G. Koziel , Nalini M. Desai , Kelly S. Lewis , Vance C. Kramer , Gregory W. Warren , Stephen V. Evola , Lyle D. Crossland , Martha S. Wright , Ellis J. Merlin , Karen L. Launis , Steven J. Rothstein , Cindy G. Bowman , John L. Dawson , Erik M. Dunder , Gary M. Pace , Janet L. Suttie , Nadine Carozzi , Annick De Framond , James O. Linder , Robert L. Miller , Bruce W. Skillings , Alan W. Mousel , Albert R. Hornbrook , Christopher P. Clucas , Moez Rajabali Meghji , Andreas H. Tanner , Francis E. Cassagne , Gilles Pollini , Terry Ray Colbert , Francis P. Cammack
IPC分类号: A01H500
CPC分类号: C12N9/12 , C07K14/325 , C12N9/88 , C12N15/8286 , Y02A40/162
摘要: Methods for transformation of maize with nucleic acid sequences of interest are disclosed. The method involves subjecting immature zygotic embryos or Type I callus to high velocity microprojectile bombardment. The method is capable of producing transformed maize lines of commercial importance and their hybrid combinations.
摘要翻译: 公开了用感兴趣的核酸序列转化玉米的方法。 该方法包括使未成熟的合子胚或I型愈伤组织进行高速微粒轰击。 该方法能够生产具有商业重要性的转化玉米品系及其杂交组合。