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公开(公告)号:US20130196322A1
公开(公告)日:2013-08-01
申请号:US13754631
申请日:2013-01-30
申请人: Michael J. Domanico , Hatim Allawi , Graham P. Lidgard , Brian Aizenstein , Oliver Hunt , Tobias Charles Zutz
发明人: Michael J. Domanico , Hatim Allawi , Graham P. Lidgard , Brian Aizenstein , Oliver Hunt , Tobias Charles Zutz
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6806 , C12Q1/6851 , C12Q2600/154 , C12Q2523/113 , C12Q2523/125 , C12Q2527/125 , C12Q2527/137
摘要: Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfate conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads.
摘要翻译: 本文提供了与DNA的化学修饰和纯化有关的技术。 具体地,该技术提供了对少量单链,片段化的DNA进行硫酸氢盐转化反应并在磁珠上进行随后的脱磺化和纯化步骤的方法。