公开(公告)号：US20140166485A1

公开(公告)日：2014-06-19

申请号：US14114688

申请日：2012-05-02

申请人： Michael J. Sailor ,  Michelle Y. Chen

发明人： Michael J. Sailor ,  Michelle Y. Chen

IPC分类号： G01N27/447

CPC分类号： G01N27/447

摘要： Electroadsorption and charged based biomolecule separation, concentration and detection with porous biosensors. In preferred embodiments, a potential is applied to a porous electrode to separate and concentrate molecules from solution. The bimolecular analytes are captured by the porous electrode itself, the same electrode that is used to generate the electric field for electroadsorption. In additional preferred embodiments, pH of the solution is adjusted to separate and concentrate biomolecules. Setting the pH equal to the protein isoelectric point was determined by the inventors to maximize concentration of biomolecules into the porous biosensor. The methods include simultaneously optically detecting charged molecules captured by the porous electrode. Methods of the invention are benign to biomolecules of interest, which are demonstrated to retain a high percentage of their activity after being released from the biosensor. Methods of the invention provide label-free detection. Advantageously, small voltages and ultrasmall volumes of solution are used in methods of the invention.

摘要翻译： 电吸收和带电的生物分子分离，浓度和多孔生物传感器的检测。 在优选的实施方案中，将电位施加到多孔电极以从溶液中分离和浓缩分子。 双分子分析物被多孔电极本身捕获，这是用于产生电吸附电场的相同电极。 在另外的优选实施方案中，调节溶液的pH以分离和浓缩生物分子。 通过发明人确定等于蛋白质等电点的pH以使生物分子的浓度最大化到多孔生物传感器中。 所述方法包括同时光学检测由多孔电极捕获的带电分子。 本发明的方法对于感兴趣的生物分子是良性的，其被证明在从生物传感器释放后保留其高活性百分比。 本发明的方法提供无标记检测。 有利地，在本发明的方法中使用小电压和超小体积的溶液。