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    F3W variants of the lantibiotic mersacidin and its use
    1.
    发明授权
    F3W variants of the lantibiotic mersacidin and its use 失效
    羊毛抗生素美沙芬的F3W变体及其用途

    公开(公告)号：US07592308B2

    公开(公告)日：2009-09-22

    申请号：US11526225

    申请日：2006-09-25

    申请人： Michael John Dawson ,  Jesus Cortes Bargallo ,  Antony Nicholas Appleyard ,  Brian Arthur Michael Rudd ,  Steven Boakes ,  Gabriele Bierbaum ,  Anja Hoffmann ,  Stephanie Schmitz

    发明人： Michael John Dawson ,  Jesus Cortes Bargallo ,  Antony Nicholas Appleyard ,  Brian Arthur Michael Rudd ,  Steven Boakes ,  Gabriele Bierbaum ,  Anja Hoffmann ,  Stephanie Schmitz

    IPC分类号： A61K38/00

    CPC分类号： C07K14/32 ,  A61K38/00

    摘要： The present invention relates to methods and products for improved production of the lantibiotic mersacidin. There is provided a method of producing a mersacidin variant which comprises introducing into a cell which is a ΔMrsA host cell an expression vector encoding said variant, and recovering said variant from the cell culture. Preferred variants include the novel variants of Table 1, e.g. mersacidin F3W, said mersacidin F3W optionally having a further one or more variations, e.g. mersacidin F3W G8A. Also provided is a Bacillus cell which is a SigH deficient strain of Bacillus sp. HIL Y-85,54728 (NCIMB Accession Number NCIMB 41211); this cell may also be used in the production of mersacidin and its variants. Further aspects of the invention include a DNA cassette which comprises a nucleotide sequence encoding the mersacidin mrsA propeptide, and a method to transform a Bacillus HIL host cell with plasmid, which method includes the step of electroporation.

    摘要翻译： 本发明涉及用于改善羊毛硫抗生素美沙芬生产的方法和产品。 提供了一种制备美沙西汀变体的方法，其包括将作为DeltaMrsA宿主细胞的细胞引入编码所述变体的表达载体，并从细胞培养物中回收所述变体。 优选的变体包括表1的新变体，例如。 美沙虫素F3W，所述美沙奎芬F3W可选地具有另外一个或多个变体，例如， mersacidin F3W G8A。 还提供了作为芽孢杆菌属SigH缺陷型菌株的芽孢杆菌细胞。 HIL Y-85,54728（NCIMB登录号NCIMB 41211）; 该细胞也可以用于制备美沙沙坦及其变体。 本发明的其它方面包括含有编码美沙肽mrsA前肽的核苷酸序列的DNA盒，以及用质粒转化芽孢杆菌HIL宿主细胞的方法，该方法包括电穿孔步骤。