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公开(公告)号：US07592308B2
公开(公告)日：2009-09-22
申请号：US11526225
申请日：2006-09-25
申请人： Michael John Dawson , Jesus Cortes Bargallo , Antony Nicholas Appleyard , Brian Arthur Michael Rudd , Steven Boakes , Gabriele Bierbaum , Anja Hoffmann , Stephanie Schmitz
发明人： Michael John Dawson , Jesus Cortes Bargallo , Antony Nicholas Appleyard , Brian Arthur Michael Rudd , Steven Boakes , Gabriele Bierbaum , Anja Hoffmann , Stephanie Schmitz
IPC分类号： A61K38/00
摘要： The present invention relates to methods and products for improved production of the lantibiotic mersacidin. There is provided a method of producing a mersacidin variant which comprises introducing into a cell which is a ΔMrsA host cell an expression vector encoding said variant, and recovering said variant from the cell culture. Preferred variants include the novel variants of Table 1, e.g. mersacidin F3W, said mersacidin F3W optionally having a further one or more variations, e.g. mersacidin F3W G8A. Also provided is a Bacillus cell which is a SigH deficient strain of Bacillus sp. HIL Y-85,54728 (NCIMB Accession Number NCIMB 41211); this cell may also be used in the production of mersacidin and its variants. Further aspects of the invention include a DNA cassette which comprises a nucleotide sequence encoding the mersacidin mrsA propeptide, and a method to transform a Bacillus HIL host cell with plasmid, which method includes the step of electroporation.
摘要翻译： 本发明涉及用于改善羊毛硫抗生素美沙芬生产的方法和产品。 提供了一种制备美沙西汀变体的方法，其包括将作为DeltaMrsA宿主细胞的细胞引入编码所述变体的表达载体，并从细胞培养物中回收所述变体。 优选的变体包括表1的新变体，例如。 美沙虫素F3W，所述美沙奎芬F3W可选地具有另外一个或多个变体，例如， mersacidin F3W G8A。 还提供了作为芽孢杆菌属SigH缺陷型菌株的芽孢杆菌细胞。 HIL Y-85,54728（NCIMB登录号NCIMB 41211）; 该细胞也可以用于制备美沙沙坦及其变体。 本发明的其它方面包括含有编码美沙肽mrsA前肽的核苷酸序列的DNA盒，以及用质粒转化芽孢杆菌HIL宿主细胞的方法，该方法包括电穿孔步骤。
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2.发明申请Production of mersacidin and its variants in SigH and/or mrsA negative bacillus host cells 失效在SigH和/或mrsA阴性杆菌宿主细胞中制备美沙奎因及其变体公开(公告)号：US20070117749A1
公开(公告)日：2007-05-24
申请号：US11526225
申请日：2006-09-25
申请人： Michael Dawson , Jesus Cortes Bargallo , Antony Appleyard , Brian Rudd , Steven Boakes , Gabriele Bierbaum , Anja Hoffmann , Stephanie Schmitz
发明人： Michael Dawson , Jesus Cortes Bargallo , Antony Appleyard , Brian Rudd , Steven Boakes , Gabriele Bierbaum , Anja Hoffmann , Stephanie Schmitz
摘要： The present invention relates to methods and products for improved production of the lantibiotic mersacidin. There is provided a method of producing a mersacidin variant which comprises introducing into a cell which is a ΔMrsA host cell an expression vector encoding said variant, and recovering said variant from the cell culture. Preferred variants include the novel variants of Table 1, e.g. mersacidin F3W, said mersacidin F3W optionally having a further one or more variations, e.g. mersacidin F3W G8A. Also provided is a Bacillus cell which is a SigH deficient strain of Bacillus sp. HIL Y-85,54728 (NCIMB Accession Number NCIMB 41211); this cell may also be used in the production of mersacidin and its variants. Further aspects of the invention include a DNA cassette which comprises a nucleotide sequence encoding the mersacidin mrsA propeptide, and a method to transform a Bacillus HIL host cell with plasmid, which method includes the step of electroporation.
摘要翻译： 本发明涉及用于改善羊毛硫抗生素美沙芬生产的方法和产品。 提供了一种制备美沙西汀变体的方法，其包括将作为DeltaMrsA宿主细胞的细胞引入编码所述变体的表达载体，并从细胞培养物中回收所述变体。 优选的变体包括表1的新变体，例如。 美沙虫素F3W，所述美沙奎芬F3W可选地具有另外一个或多个变体，例如， mersacidin F3W G8A。 还提供了作为芽孢杆菌属SigH缺陷型菌株的芽孢杆菌细胞。 HIL Y-85,54728（NCIMB登录号NCIMB 41211）; 该细胞也可以用于制备美沙沙坦及其变体。 本发明的其它方面包括含有编码美沙肽mrsA前肽的核苷酸序列的DNA盒，以及用质粒转化芽孢杆菌HIL宿主细胞的方法，该方法包括电穿孔步骤。
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3.发明申请LANTIBIOTIC BIOSYNTHETIC GENE CLUSTERS FROM A. GARBADINENSIS AND A. LIGURIAE 失效来自A.GARBADINENSIS和A.LIGURIAE的生物生物学基因组公开(公告)号：US20110306091A1
公开(公告)日：2011-12-15
申请号：US13157454
申请日：2011-06-10
CPC分类号： C07K14/365 , C12R1/045
摘要： This invention relates to characterisation of the biosynthetic gene cluster for the lantibiotic actagardine, identification of a novel variant of actagardine and its biosynthetic cluster, and methods of production and use of actagardine, a novel actagardine variant, herein referred to as actagardine B, and variants of both of these produced according to this invention, utilizing genes from the characterised biosynthetic gene clusters.
摘要翻译： 本发明涉及用于拉长肽抗生素的生物合成基因簇的鉴定，阿加瓜定及其生物合成簇的新型变体的鉴定，以及生产和使用阿卡格胺的方法，一种新颖的阿格达肽变体，在本文中称为卡那加丁B，以及变异体 根据本发明生产的两种，利用来自特征性生物合成基因簇的基因。
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4.发明授权Lantibiotic biosynthetic gene clusters from A. garbadinensis and A. Liguriae 有权来自A. garbadinensis和A.Liguriae的Lantibiotic生物合成基因簇公开(公告)号：US07989416B2
公开(公告)日：2011-08-02
申请号：US12161221
申请日：2007-01-17
CPC分类号： C07K14/365 , C12R1/045
摘要： Characterization of the biosynthetic gene cluster for the lantibiotic actagardine, identification of a novel variant of actagardine and its biosynthetic cluster, and methods of production and use of actagardine are described.
摘要翻译： 描述了羊毛硫抗生素卡氨酸的生物合成基因簇的鉴定，阿加瓜定及其生物合成簇的新变体的鉴定，以及阿加胍的生产和使用方法。
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5.发明申请LANTIBIOTIC BIOSYNTHETIC GENE CLUSTERS FROM A. GARBADINENSIS AND A. LIGURIAE 有权来自A.GARBADINENSIS和A.LIGURIAE的生物生物学基因组公开(公告)号：US20100048459A1
公开(公告)日：2010-02-25
申请号：US12161221
申请日：2007-01-17
CPC分类号： C07K14/365 , C12R1/045
摘要： This invention relates to characterisation of the biosynthetic gene cluster for the lantibiotic actagardine, identification of a novel variant of actagardine and its biosynthetic cluster, and methods of production and use of actagardine, a novel actagardine variant, herein referred to as actagardine B, and variants of both of these produced according to this invention, utilizing genes from the characterised biosynthetic gene clusters.
摘要翻译： 本发明涉及用于拉长肽抗生素的生物合成基因簇的鉴定，阿加瓜定及其生物合成簇的新型变体的鉴定，以及生产和使用阿卡格胺的方法，一种新颖的阿格达肽变体，在本文中称为卡那加丁B，以及变异体 根据本发明生产的两种，利用来自特征性生物合成基因簇的基因。
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6.发明授权Lantibiotic biosynthetic gene clusters from A. garbadinensis and A. liguriae 失效来自加尔丁（A. Garbadinensis）和百里香肠杆菌（A.tururiae）的Lantibiotic生物合成基因簇公开(公告)号：US08465947B2
公开(公告)日：2013-06-18
申请号：US13157454
申请日：2011-06-10
CPC分类号： C07K14/365 , C12R1/045
摘要： Characterization of the biosynthetic gene cluster for the lantibiotic antibiotic actagardine, identification of a novel variant of actagardine and its biosynthetic cluster, and methods of production and use of actagardine, a novel actagardine variant, herein referred to as actagardine B, and variants of both of these produced according to this invention, utilizing genes from the characterized biosynthetic gene clusters, is described.
摘要翻译： 用于羊毛硫抗生素抗生素卡肽的生物合成基因簇的鉴定，阿加瓜定及其生物合成簇的新型变体的鉴定，以及生产和使用阿格达肽（一种新颖的阿格达肽变体）的方法，本文称为卡那加丁B，以及两者的变体 描述了根据本发明产生的利用表征的生物合成基因簇的基因的这些。
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