公开(公告)号：US20080233563A1

公开(公告)日：2008-09-25

申请号：US10590891

申请日：2005-02-25

申请人： Michael Mitas ,  David J. Cole ,  William E. Gillanders ,  Kaidi Mikhitarian

发明人： Michael Mitas ,  David J. Cole ,  William E. Gillanders ,  Kaidi Mikhitarian

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/6886 ,  C12Q2600/158

摘要： The present invention provides truncated gene-specific primers in panels that can be used during the reverse transcription step of RT-PCR to increase signal detection of cancer gene markers in a tissue sample. Also provided are forward and reverse primers for RT-PCR. Methods of using the primers are also provided.

摘要翻译： 本发明提供了可在RT-PCR的逆转录步骤期间使用的片段中的截短的基因特异性引物，以增加组织样品中癌症基因标记的信号检测。 还提供了用于RT-PCR的正向和反向引物。 还提供了使用引物的方法。

公开(公告)号：US07981616B2

公开(公告)日：2011-07-19

申请号：US10590891

申请日：2005-02-25

申请人： Michael Mitas ,  David J. Cole ,  William E. Gillanders ,  Kaidi Mikhitarian

发明人： Michael Mitas ,  David J. Cole ,  William E. Gillanders ,  Kaidi Mikhitarian

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/158

摘要： The present invention provides truncated gene-specific primers in panels that can be used during the reverse transcription step of RT-PCR to increase signal detection of cancer gene markers in a tissue sample. Also provided are forward and reverse primers for RT-PCR. Methods of using the primers are also provided.

摘要翻译： 本发明提供了可在RT-PCR的逆转录步骤期间使用的片段中的截短的基因特异性引物，以增加组织样品中癌症基因标记的信号检测。 还提供了用于RT-PCR的正向和反向引物。 还提供了使用引物的方法。

公开(公告)号：US07824857B2

公开(公告)日：2010-11-02

申请号：US11003223

申请日：2004-12-02

申请人： Michael Mitas ,  David J. Cole ,  William E. Gillanders ,  Michael Wallace

发明人： Michael Mitas ,  David J. Cole ,  William E. Gillanders ,  Michael Wallace

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118

摘要： Provided is a method for detecting metastases of epithelial cancers, comprising detecting in non-primary tissue overexpression of a nucleic acid of KS1/4, or detecting in non-primary tissue overexpression of a combination of nucleic acids of KS1/4 and PIP, of nucleic acids of KS1/4 and mam, of nucleic acids of PIP and mam, of nucleic acids of KS1/4, PIP and mam, or of nucleic acids of KS1/4 and lunx, the overexpression of a nucleic acid of KS1/4, or the overexpression of a combination of nucleic acids of KS1/4 and PIP, of nucleic acids of KS1/4 and mam, of nucleic acids of PIP and mam, of nucleic acids of KS1/4, PIP and mam, or of nucleic acids of KS1/4 and lunx in non-primary tissue being correlated with metastases of epithelial cancers.

摘要翻译： 提供了一种用于检测上皮癌转移的方法，包括在非原代组织中检测KS1 / 4核酸的过表达，或检测非小细胞组织过表达KS1 / 4和PIP核酸的组合， KS1 / 4，PIP和mam核酸或KS1 / 4和lunx核酸的PIP和mam的核酸的KS1 / 4和mam的核酸，KS1 / 4的核酸的过表达 或KS1 / 4，PIP和mam核酸或核酸的核酸的PIP和mam的核酸的KS1 / 4和mam的核酸的KS1 / 4和PIP的核酸的组合的过表达或核酸 KS1 / 4的酸和非原发性组织中的lunx与上皮癌的转移相关。

公开(公告)号：US20080241849A1

公开(公告)日：2008-10-02

申请号：US12152423

申请日：2008-05-13

申请人： Michael Mitas ,  David J. Cole ,  William E. Gillanders ,  Michael Wallace

发明人： Michael Mitas ,  David J. Cole ,  William E. Gillanders ,  Michael Wallace

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118

摘要： Provided is a method for detecting metastases of epithelial cancers, comprising detecting in non-primary tissue overexpression of a nucleic acid of KS1/4, or detecting in non-primary tissue overexpression of a combination of nucleic acids of KS1/4 and PIP, of nucleic acids of KS1/4 and mam, of nucleic acids of PIP and mam, of nucleic acids of KS1/4, PIP and mam, or of nucleic acids of KS1/4 and lunx, the overexpression of a nucleic acid of KS1/4, or the overexpression of a combination of nucleic acids of KS1/4 and PIP, of nucleic acids of KS1/4 and mam, of nucleic acids of PIP and mam, of nucleic acids of KS1/4, PIP and mam, or of nucleic acids of KS1/4 and lunx in non-primary tissue being correlated with metastases of epithelial cancers.

摘要翻译： 提供了一种用于检测上皮癌转移的方法，包括在非原代组织中检测KS1 / 4核酸的过表达，或检测非小细胞组织过表达KS1 / 4和PIP核酸的组合， KS1 / 4，PIP和mam核酸或KS1 / 4和lunx核酸的PIP和mam的核酸的KS1 / 4和mam的核酸，KS1 / 4的核酸的过表达 或KS1 / 4，PIP和mam核酸或核酸的核酸的PIP和mam的核酸的KS1 / 4和mam的核酸的KS1 / 4和PIP的核酸的组合的过表达或核酸 KS1 / 4的酸和非原发性组织中的lunx与上皮癌的转移相关。

公开(公告)号：US20050123976A1

公开(公告)日：2005-06-09

申请号：US11003223

申请日：2004-12-02

申请人： Michael Mitas ,  David Cole ,  William Gillanders ,  Michael Wallace

发明人： Michael Mitas ,  David Cole ,  William Gillanders ,  Michael Wallace

IPC分类号： A61B20060101 ,  C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118

摘要： Provided is a method for detecting metastases of epithelial cancers, comprising detecting in non-primary tissue overexpression of a nucleic acid of KS1/4, or detecting in non-primary tissue overexpression of a combination of nucleic acids of KS1/4 and PIP, of nucleic acids of KS1/4 and mam, of nucleic acids of PIP and mam, of nucleic acids of KS1/4, PIP and mam, or of nucleic acids of KS1/4 and lunx, the overexpression of a nucleic acid of KS1/4, or the overexpression of a combination of nucleic acids of KS1/4 and PIP, of nucleic acids of KS1/4 and mam, of nucleic acids of PIP and mam, of nucleic acids of KS1/4, PIP and mam, or of nucleic acids of KS1/4 and lunx in non-primary tissue being correlated with metastases of epithelial cancers.

摘要翻译： 提供了一种用于检测上皮癌转移的方法，包括在非原代组织中检测KS1 / 4核酸的过表达，或检测非小细胞组织过表达KS1 / 4和PIP核酸的组合， KS1 / 4，PIP和mam核酸或KS1 / 4和lunx核酸的PIP和mam的核酸的KS1 / 4和mam的核酸，KS1 / 4的核酸的过表达 或KS1 / 4，PIP和mam核酸或核酸的核酸的PIP和mam的核酸的KS1 / 4和mam的核酸的KS1 / 4和PIP的核酸的组合的过表达或核酸 KS1 / 4的酸和非原发性组织中的lunx与上皮癌的转移相关。

公开(公告)号：US20080026481A1

公开(公告)日：2008-01-31

申请号：US11820092

申请日：2007-06-18

申请人： Michael Mitas

发明人： Michael Mitas

IPC分类号： G01N33/00

CPC分类号： G01N33/57423

摘要： Provided is a method of identifying a subject at risk of recurrence of adenocarcinoma, comprising detecting markers associated with recurrence. Provided is a method of identifying a subject at risk for recurrence of adenocarcinoma, comprising determining the ratio of EpCAM2 to CK19 in primary adenocarcinoma tissue from the subject, a high ratio of EpCAM2 to CK19 M indicating a subject at risk for recurrence. Also provided is method of identifying a subject at risk for recurrence of adenocarcinoma, comprising determining the ratio of CK19 and P-cadherin in primary adenocarcinoma tissue from the subject, a low ratio of CK19 to P-cadherin indicating a subject at risk for recurrence. A method of identifying a subject at risk for recurrence of adenocarcinoma is provided, comprising determining the ratio of Map7 to EpCAM2 in primary adenocarcinoma tissue from the subject, a low ratio of Map7 to EpCAM2 indicating a subject at risk for recurrence. Provided is a method of identifying a subject at risk for recurrence of adenocarcinoma, comprising determining the ratio of P-cadherin to E-cadherin in primary adenocarcinoma tissue from the subject, a high ratio of P-cadherin to E-cadherin indicating a subject at risk for recurrence.

摘要翻译： 本发明提供了鉴定患有腺癌复发风险的受试者的方法，其包括检测与复发相关的标志物。 本发明提供了鉴定患有腺癌复发风险的受试者的方法，包括确定来自受试者的原发性腺癌组织中EpCAM2与CK19的比例，EpCAM2与CK19M的高比例，表明患有复发风险的受试者。 还提供了鉴定患有腺癌复发风险的受试者的方法，包括测定受试者原发性腺癌组织中CK19和P-钙粘蛋白的比例，CK19与P-钙粘蛋白的比例低，表明患有复发风险的受试者。 提供了鉴定患有腺癌复发风险的受试者的方法，包括测定来自受试者的原发性腺癌组织中Map7与EpCAM2的比例，Map7与EpCAM2的比例低，表明存在复发风险的受试者。 本发明提供一种识别患有腺癌复发风险的受试者的方法，包括测定受试者的原发性腺癌组织中P-钙粘蛋白与E-钙粘蛋白的比例，P-钙粘蛋白与E-钙粘蛋白的比例高， 复发风险。

公开(公告)号：US20070231822A1

公开(公告)日：2007-10-04

申请号：US11711493

申请日：2007-02-27

申请人： Michael Mitas

发明人： Michael Mitas

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/118

摘要： Methods are provided for the detection of and determining prognosis of metastatic breast, lung, prostate, and/or pancreatic cancer using various genetic markers, including markers for gene clusters linked by Esx. In one method, breast cancer micrometastases and non-small cell lung cancer metastases or micrometastases are detected in a patient by determining whether the AGR2 or TFF1 genes are overexpressed in a cell sample compared to control lymph node tissue cells. In a further method, the likelihood that a patient diagnosed with breast cancer will respond to hormonal therapy is predicted by determining a higher expression level of the AGR2 gene compared to a control gene. In a further method, a decreased probability of survival for a patient diagnosed with early stage non-small cell lung cancer is predicted by determining a higher expression level of the AGR2 gene compared to a control gene. Kits for practicing the methods of the invention are further provided. Methods are also provided for the identification of markers for which overexpression is indicative of the presence of micrometastatic disease.

摘要翻译： 提供了使用各种遗传标记（包括由Esx连接的基因簇的标志物）来检测和确定转移性乳腺，肺，前列腺和/或胰腺癌的预后的方法。 在一种方法中，与对照淋巴结组织细胞相比，通过确定AGR2或TFF1基因是否在细胞样品中过表达，在患者体内检测到乳腺癌微转移和非小细胞肺癌转移或微转移。 在另一种方法中，通过确定AGR2基因与对照基因相比较高的表达水平来预测诊断患有乳腺癌的患者对激素治疗作出反应的可能性。 在另一种方法中，通过确定AGR2基因与对照基因相比较高的表达水平来预测诊断为早期非小细胞肺癌的患者的生存概率降低。 还提供了用于实施本发明的方法的试剂盒。 还提供了用于鉴定过表达指示存在微转移性疾病的标记物的方法。