公开(公告)号：US20230184751A1

公开(公告)日：2023-06-15

申请号：US17925119

申请日：2021-05-14

Applicant: Ming-Che SHIH ,  ACADEMIA SINICA

Inventor： Wen-Bin YANG

IPC: G01N33/53 ,  G01N30/72 ,  G01N33/58

CPC classification number: G01N33/5308 ,  G01N30/7233 ,  G01N33/58 ,  G01N2030/027

Abstract: The present invention relates to a system and method for carbohydrate analysis in a flow chemistry manner. The present invention at least features continuous reactions of glycan hydrolysis in combination with saccharide labeling which is helpful to improve the existing approaches in glycan structural analysis.

公开(公告)号：US20170299530A1

公开(公告)日：2017-10-19

申请号：US15640757

申请日：2017-07-03

Applicant: ACADEMIA SINICA

Inventor： Wen-Bin YANG

IPC: G01N24/08 ,  G01N33/02 ,  G01N33/14 ,  G01N33/50

CPC classification number: G01N24/087 ,  C07D233/64 ,  C07D241/44 ,  C07H3/02 ,  C07H7/06 ,  C07H13/02 ,  G01N33/025 ,  G01N33/143 ,  G01N33/50 ,  G01N33/58 ,  G01N2400/00 ,  G01N2458/00 ,  G01N2458/15

Abstract: Provided is a method for quantitating a saccharide in a liquid sample. The method comprises incubating the liquid sample with 2,3-naphthalenediamine in the presence of iodine to allow a naphthimidazole group to be linked to the saccharide to obtain a first mixture; obtaining an 1H-NMR spectrum of the first mixture; and comparing, in said 1H-NMR spectrum, the intensity or integral of a proton signal corresponding to the saccharide to the intensity or integral of a proton signal corresponding to an internal standard present in the first mixture.

公开(公告)号：US20150168415A1

公开(公告)日：2015-06-18

申请号：US14569368

申请日：2014-12-12

Applicant: ACADEMIA SINICA

Inventor： Wen-Bin YANG

IPC: G01N33/58 ,  C07K1/107 ,  C07D241/52 ,  C07D233/64 ,  C07H15/26

CPC classification number: C07D241/52 ,  C07D233/64 ,  C07H15/26 ,  C07K1/13 ,  G01N33/58 ,  G01N2400/00 ,  G01N2458/00

Abstract: Provided is an isotope-labelled compound having a general formula IIa′, S-Y′-BIM, wherein S is a sugar moiety, Y′ is an isotope-containing sensor moiety and BIM is a benzimidazole. The compound may be used as an agent for saccharide analysis by magnetic resonance spectroscopy (NMR). A new glycan sequencing method and a glycoprotein synthesis method by using these compounds are also provided.

Abstract translation: 提供具有通式IIa'，S-Y'-BIM的同位素标记的化合物，其中S是糖部分，Y'是含同位素的传感器部分，BIM是苯并咪唑。 该化合物可以用作通过磁共振光谱（NMR）进行糖分析的试剂。 还提供了使用这些化合物的新的聚糖测序方法和糖蛋白合成方法。