公开(公告)号：US20250011798A1

公开(公告)日：2025-01-09

申请号：US18702284

申请日：2022-10-17

Applicant: ModernaTX, Inc.

Inventor： Kevin Smith ,  Joe R. Sarkis ,  Stoil Dimitrov

IPC: C12N15/70 ,  C12P19/34

Abstract: Provided herein are genetically modified microorganisms comprising a nonsense mutation in a gene encoding an efflux pump or import protein, and a vector encoding a suppressor tRNA that enables translation of the efflux pump or import protein, mitigating the effect of the nonsense mutation. Also provided are methods of producing a vector or markerless DNA using the genetically modified microorganisms provided herein.

公开(公告)号：US11485960B2

公开(公告)日：2022-11-01

申请号：US17325883

申请日：2021-05-20

Applicant: ModernaTX, Inc.

Inventor： Athanasios Dousis ,  Kanchana Ravichandran ,  Amy E. Rabideau ,  Margaret Franklin ,  Kevin Smith ,  Michelle Lynn Hall

IPC: C12N9/12 ,  C12P19/34

Abstract: The present disclosure provides RNA polymerase variants for high efficiency transcription.

公开(公告)号：US20210309976A1

公开(公告)日：2021-10-07

申请号：US17325883

申请日：2021-05-20

Applicant: ModernaTX, Inc.

Inventor： Athanasios Dousis ,  Kanchana Ravichandran ,  Amy E. Rabideau ,  Margaret Franklin ,  Kevin Smith

IPC: C12N9/12 ,  C12P19/34

Abstract: The present disclosure provides RNA polymerase variants for high efficiency transcription.

公开(公告)号：US20250084397A1

公开(公告)日：2025-03-13

申请号：US18725999

申请日：2022-11-04

Applicant: MODERNATX, INC.

Inventor： Kevin Smith

IPC: C12N15/10 ,  C12N9/22

Abstract: Provided herein are methods of purifying nucleic acids (e.g., DNA) for gene synthesis using combinations of nucleases. Also provided are improved products for use in the production of RNA.

公开(公告)号：US20230287437A1

公开(公告)日：2023-09-14

申请号：US18008139

申请日：2021-06-03

Applicant: ModernaTX, Inc.

Inventor： Kevin Smith ,  Marcus Duvall ,  Bhargav Tilak

IPC: C12N15/70

CPC classification number: C12N15/70 ,  C12N2800/22 ,  C12N2800/101 ,  C12N2800/50 ,  C12N2820/005

Abstract: Compositions for the production of plasmid nucleic acids and methods of making and using the same are provided.

公开(公告)号：US20250051736A1

公开(公告)日：2025-02-13

申请号：US18702280

申请日：2022-10-17

Applicant: ModernaTX, Inc.

Inventor： Kevin Smith

IPC: C12N9/12 ,  C12N15/70

Abstract: Described here are genetically modified microorganisms with reduced protease activity for the expression of recombinant proteins and without mucoid phenotypes. Also described are methods of making and using the same.

公开(公告)号：US12180518B2

公开(公告)日：2024-12-31

申请号：US17816696

申请日：2022-08-01

Applicant: ModernaTX, Inc.

Inventor： Athanasios Dousis ,  Kanchana Ravichandran ,  Amy E. Rabideau ,  Margaret Franklin ,  Kevin Smith ,  Michelle Lynn Hall

IPC: C12N9/12 ,  C12P19/34

Abstract: The present disclosure provides RNA polymerase variants for high efficiency transcription.