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公开(公告)号：US20200165629A1
公开(公告)日：2020-05-28
申请号：US16063874
申请日：2016-12-20
IPC分类号： C12N15/86
摘要： The present invention relates to the field of the production of lentiviral vectors (LV) for gene therapy. More particularly, the invention relates to a system to obtain the stable integration of a Self Inactivating (SIN) lentiviral transfer vector into a packaging cell line. The SIN lentiviral transfer vector is integrated using a DNA fragment containing the Inverted Terminal Repeats (ITR) of Adeno Associated Virus (AAV), thus obtaining the stable producer cell line for SIN lentiviral vectors.
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公开(公告)号：US20180002721A1
公开(公告)日：2018-01-04
申请号：US15226762
申请日：2016-08-02
申请人： MolMed, S.p.A.
IPC分类号： C12N15/86 , C12N15/867 , C07K14/54
CPC分类号： C12N15/86 , A61K38/00 , A61K39/21 , A61K2039/525 , C07K14/005 , C07K14/54 , C07K16/10 , C12N15/8673 , C12N2710/14144 , C12N2740/16052 , C12N2740/16122 , C12N2740/16134 , C12N2750/14143 , C12N2750/14352 , C12N2800/40 , C12N2800/50 , C12N2810/6054
摘要： The present invention provides new stable packaging cell lines and producer cell lines as well as methods to obtain them, and a new method to produce lentiviral vectors using such cell lines. New methods and packaging cell lines of the invention are generated using a baculo-AAV hybrid system for stable expression of structural and regulatory lentiviral proteins, such system comprising a baculoviral backbone containing an integration cassette flanked by AAV ITR, in combination with a plasmid encoding rep protein. This system allows to obtain a stable integration of the structural and regulatory HIV-1 proteins gag/pol and rev. The system allows to obtain a first intermediate including only the structural and regulatory HIV proteins gag/pol and rev, to be used as starting point to obtain stable packaging cell lines as well as producer cell lines.
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