公开(公告)号：US20180169148A1

公开(公告)日：2018-06-21

申请号：US15545555

申请日：2016-01-21

申请人： Fred Hutchinson Cancer Research Center

发明人： Jennifer E. Adair ,  Hans-Peter Kiem

IPC分类号： A61K35/28 ,  A61P35/00 ,  C12N15/867 ,  C12Q1/68 ,  C12M1/42

CPC分类号： A61K35/28 ,  A61P35/00 ,  C12M35/02 ,  C12M47/04 ,  C12N15/8673 ,  C12N2740/16043 ,  C12Q1/68

摘要： A platform for ex vivo isolation, production, and formulation of genetically-modified cells is described. The platform utilizes a software-enabled point-of-care and/or portable device making gene therapy more widely available.

公开(公告)号：US20180002721A1

公开(公告)日：2018-01-04

申请号：US15226762

申请日：2016-08-02

申请人： MolMed, S.p.A.

发明人： Chiara BOVOLENTA ,  Anna Stornaiuolo ,  Paolo Rizzardi ,  Fulvio Mavilio

IPC分类号： C12N15/86 ,  C12N15/867 ,  C07K14/54

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K39/21 ,  A61K2039/525 ,  C07K14/005 ,  C07K14/54 ,  C07K16/10 ,  C12N15/8673 ,  C12N2710/14144 ,  C12N2740/16052 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2750/14143 ,  C12N2750/14352 ,  C12N2800/40 ,  C12N2800/50 ,  C12N2810/6054

摘要： The present invention provides new stable packaging cell lines and producer cell lines as well as methods to obtain them, and a new method to produce lentiviral vectors using such cell lines. New methods and packaging cell lines of the invention are generated using a baculo-AAV hybrid system for stable expression of structural and regulatory lentiviral proteins, such system comprising a baculoviral backbone containing an integration cassette flanked by AAV ITR, in combination with a plasmid encoding rep protein. This system allows to obtain a stable integration of the structural and regulatory HIV-1 proteins gag/pol and rev. The system allows to obtain a first intermediate including only the structural and regulatory HIV proteins gag/pol and rev, to be used as starting point to obtain stable packaging cell lines as well as producer cell lines.

公开(公告)号：US09441245B2

公开(公告)日：2016-09-13

申请号：US13819991

申请日：2011-09-01

申请人： Chiara Bovolenta ,  Anna Stornaiuolo ,  Paolo Rizzardi ,  Fulvio Mavilio

发明人： Chiara Bovolenta ,  Anna Stornaiuolo ,  Paolo Rizzardi ,  Fulvio Mavilio

IPC分类号： C12N15/86 ,  C12N15/867 ,  C07K14/54 ,  C07K14/005 ,  A61K39/21 ,  A61K39/00 ,  C07K16/10 ,  A61K38/00

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K39/21 ,  A61K2039/525 ,  C07K14/005 ,  C07K14/54 ,  C07K16/10 ,  C12N15/8673 ,  C12N2710/14144 ,  C12N2740/16052 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2750/14143 ,  C12N2750/14352 ,  C12N2800/40 ,  C12N2800/50 ,  C12N2810/6054

摘要： The present invention provides new stable packaging cell lines and producer cell lines as well as methods to obtain them, and a new method to produce lentiviral vectors using such cell lines. New methods and packaging cell lines of the invention are generated using a baculo-AAV hybrid system for stable expression of structural and regulatory lentiviral proteins, such system comprising a baculoviral backbone containing an integration cassette flanked by AAV ITR, in combination with a plasmid encoding rep protein. This system allows to obtain a stable integration of the structural and regulatory HIV-1 proteins gag/pol and rev. The system allows to obtain a first intermediate including only the structural and regulatory HIV proteins gag/pol and rev, to be used as starting point to obtain stable packaging cell lines as well as producer cell lines.

摘要翻译： 本发明提供新的稳定的包装细胞系和生产细胞系以及获得它们的方法，以及使用这种细胞系产生慢病毒载体的新方法。 本发明的新方法和包装细胞系使用杆状AAV杂交系统产生，用于结构和调节性慢病毒蛋白的稳定表达，该系统包含含有侧翼为AAV ITR的整合盒的杆状病毒主链，与编码rep 蛋白。 该系统允许获得结构和调节的HIV-1蛋白gag / pol和rev的稳定整合。 该系统允许获得仅包括结构和调节性HIV蛋白gag / pol和rev的第一个中间体，用作起始点以获得稳定的包装细胞系以及生产细胞系。

公开(公告)号：US20240336936A1

公开(公告)日：2024-10-10

申请号：US18680137

申请日：2024-05-31

申请人： LONZA WALKERSVILLE, INC.

发明人： Young SHIN ,  Anandita SETH ,  Bingnan GU

IPC分类号： C12N15/867 ,  C07K14/16 ,  C12N5/071 ,  C12N9/12 ,  C12N15/86

CPC分类号： C12N15/867 ,  C07K14/161 ,  C07K14/162 ,  C07K14/163 ,  C12N5/0686 ,  C12N9/1241 ,  C12N15/8673 ,  C12N15/8676 ,  C12N15/86 ,  C12N2740/15051 ,  C12N2740/16052 ,  C12N2760/20222

摘要： The present disclosure relates to methods for producing lentiviral vector-producing cells. Specifically the methods utilize two plasmids, rather than four, to provide the required packaging elements and transfer vector to a cell, allowing for the production of a large number of lentiviral producer cells, including suspension-based cells, and the production of high amounts of lentivirus. These methods allow for the production of cells that can be later induced to produce lentivirus, and can be tailored to include a specific gene of interest.

公开(公告)号：US20140051125A1

公开(公告)日：2014-02-20

申请号：US13819991

申请日：2011-09-01

申请人： Chiara Bovolenta ,  Anna Stornaiuolo ,  Paolo Rizzardi ,  Fulvio Mavilio

发明人： Chiara Bovolenta ,  Anna Stornaiuolo ,  Paolo Rizzardi ,  Fulvio Mavilio

IPC分类号： C12N15/86

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K39/21 ,  A61K2039/525 ,  C07K14/005 ,  C07K14/54 ,  C07K16/10 ,  C12N15/8673 ,  C12N2710/14144 ,  C12N2740/16052 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2750/14143 ,  C12N2750/14352 ,  C12N2800/40 ,  C12N2800/50 ,  C12N2810/6054

摘要： The present invention provides new stable packaging cell lines and producer cell lines as well as methods to obtain them, and a new method to produce lentiviral vectors using such cell lines. New methods and packaging cell lines of the invention are generated using a baculo-AAV hybrid system for stable expression of structural and regulatory lentiviral proteins, such system comprising a baculoviral backbone containing an integration cassette flanked by AAV ITR, in combination with a plasmid encoding rep protein. This system allows to obtain a stable integration of the structural and regulatory HIV-1 proteins gag/pol and rev. The system allows to obtain a first intermediate including only the structural and regulatory HIV proteins gag/pol and rev, to be used as starting point to obtain stable packaging cell lines as well as producer cell lines.

摘要翻译： 本发明提供新的稳定的包装细胞系和生产细胞系以及获得它们的方法，以及使用这种细胞系产生慢病毒载体的新方法。 本发明的新方法和包装细胞系使用杆状AAV杂交系统产生，用于结构和调节性慢病毒蛋白的稳定表达，该系统包含含有侧翼为AAV ITR的整合盒的杆状病毒主链，与编码rep 蛋白。 该系统允许获得结构和调节的HIV-1蛋白gag / pol和rev的稳定整合。 该系统允许获得仅包括结构和调节性HIV蛋白gag / pol和rev的第一个中间体，用作起始点以获得稳定的包装细胞系以及生产细胞系。

公开(公告)号：US12037599B2

公开(公告)日：2024-07-16

申请号：US17001114

申请日：2020-08-24

申请人： LONZA WALKERSVILLE, INC.

发明人： Young Shin ,  Anandita Seth ,  Bingnan Gu

IPC分类号： C12N15/867 ,  C07K14/16 ,  C12N5/071 ,  C12N9/12 ,  C12N15/86

CPC分类号： C12N15/867 ,  C07K14/161 ,  C07K14/162 ,  C07K14/163 ,  C12N5/0686 ,  C12N9/1241 ,  C12N15/8673 ,  C12N15/8676 ,  C12N15/86 ,  C12N2740/15051 ,  C12N2740/16052 ,  C12N2760/20222

摘要： The present disclosure relates to methods for producing lentiviral vector-producing cells. Specifically the methods utilize two plasmids, rather than four, to provide the required packaging elements and transfer vector to a cell, allowing for the production of a large number of lentiviral producer cells, including suspension-based cells, and the production of high amounts of lentivirus. These methods allow for the production of cells that can be later induced to produce lentivirus, and can be tailored to include a specific gene of interest.

公开(公告)号：US20230212263A1

公开(公告)日：2023-07-06

申请号：US18068376

申请日：2022-12-19

申请人： The University of North Carolina at Chapel Hill

发明人： Tal Kafri

IPC分类号： C12N7/02 ,  C07K14/745 ,  C12N15/867 ,  C12N9/64

CPC分类号： C12N7/025 ,  C07K14/745 ,  C12N15/8673 ,  C12N9/644 ,  C12Y304/21022 ,  C07K2319/30

摘要： The present invention provides Factor IX fusion proteins with higher specific activity and a longer useful clotting function relative to wild type or non-modified Factor IX protein.

公开(公告)号：US10072250B2

公开(公告)日：2018-09-11

申请号：US14900837

申请日：2014-07-10

申请人： TAKARA BIO INC.

发明人： Shuhei Sakamoto ,  Yasuhiro Kawano ,  Katsuyuki Dodo ,  Tatsuji Enoki ,  Hirofumi Yoshioka ,  Hikaru Takakura ,  Junichi Mineno

IPC分类号： C12N7/00 ,  C12N15/86 ,  C12N7/02 ,  C12N15/867

CPC分类号： C12N7/00 ,  C12N2750/14151 ,  C12N15/86 ,  C12N2750/14143 ,  C12N2750/14043 ,  C12N2750/14122 ,  C12N2750/14142 ,  C12N7/02 ,  C12N2750/14141 ,  C12N2750/14351 ,  C12N2800/50 ,  C12N15/8673 ,  C12N2750/14352 ,  C12N2750/14152

摘要： The present invention provides a method for efficiently manufacturing a non-enveloped virus with high purity without laborious operation by cultivating cells having the ability to produce a non-enveloped virus and bringing the cells and an acidic solution into contact with each other. A non-enveloped virus vector manufactured by the method of the present invention and a composition having the non-enveloped viral vector as an active ingredient are very useful as gene transfer methods in the fields of basic research and clinical application gene therapy.

公开(公告)号：US20160152955A1

公开(公告)日：2016-06-02

申请号：US14900837

申请日：2014-07-10

申请人： TAKARA BIO INC.

发明人： Shuhei SAKAMOTO ,  Yasuhiro KAWANO ,  Katsuyuki DODO ,  Tatsuji ENOKI ,  Hirofumi YOSHIOKA ,  Hikaru TAKAKURA ,  Junichi MINENO

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  C12N2750/14151 ,  C12N15/86 ,  C12N2750/14143 ,  C12N2750/14043 ,  C12N2750/14122 ,  C12N2750/14142 ,  C12N7/02 ,  C12N2750/14141 ,  C12N2750/14351 ,  C12N2800/50 ,  C12N15/8673 ,  C12N2750/14352 ,  C12N2750/14152

摘要： The present invention provides a method for efficiently manufacturing a non-enveloped virus with high purity without laborious operation by cultivating cells having the ability to produce a non-enveloped virus and bringing the cells and an acidic solution into contact with each other. A non-enveloped virus vector manufactured by the method of the present invention and a composition having the non-enveloped viral vector as an active ingredient are very useful as gene transfer methods in the fields of basic research and clinical application gene therapy.

摘要翻译： 本发明提供了通过培养具有产生非包膜病毒的能力的细胞并使细胞和酸性溶液相互接触的方式，高效地制造无包膜病毒的方法，而无需费力的操作。 通过本发明方法制备的非包膜病毒载体和具有非包膜病毒载体作为活性成分的组合物在基础研究和临床应用基因治疗领域中作为基因转移方法是非常有用的。

公开(公告)号：US09133481B2

公开(公告)日：2015-09-15

申请号：US13819989

申请日：2011-09-01

申请人： Chiara Bovolenta ,  Anna Stornaiuolo ,  Paolo Rizzardi ,  Fulvio Mavilio

发明人： Chiara Bovolenta ,  Anna Stornaiuolo ,  Paolo Rizzardi ,  Fulvio Mavilio

IPC分类号： C12N15/63 ,  C12N15/86 ,  C12N15/867

CPC分类号： C12N15/86 ,  C12N15/8673 ,  C12N2710/14144 ,  C12N2740/16052 ,  C12N2750/14143 ,  C12N2800/40 ,  C12N2800/50

摘要： The present invention provides a new semi-stable packaging cell line and a method to produce lentiviral vectors (LV), using the semi-stable packaging cell line. New methods and packaging cell lines of the invention are generated using a baculo-AAV hybrid system for stable expression of structural and regulatory lentiviral proteins, such system comprising a baculoviral backbone containing an integration cassette flanked by AAV ITRs, in combination with a plasmid encoding rep protein. This system allows to obtain a stable integration of the structural and regulatory HIV-1 proteins gag/pol and rev. The system allows to obtain a cell line including the structural and regulatory HIV proteins gag/pol and rev, to be used for a semi-stable LV production.

摘要翻译： 本发明提供一种新的半稳定包装细胞系和使用半稳定包装细胞系产生慢病毒载体（LV）的方法。 本发明的新方法和包装细胞系使用杆状AAV杂交系统产生，用于稳定表达结构和调节性慢病毒蛋白，该系统包含含有侧翼为AAV ITR的整合盒的杆状病毒主链，与编码rep 蛋白。 该系统允许获得结构和调节的HIV-1蛋白gag / pol和rev的稳定整合。 该系统允许获得包括用于半稳定LV生产的结构和调节性HIV蛋白gag / pol和rev的细胞系。