公开(公告)号：US20140096278A1

公开(公告)日：2014-04-03

申请号：US14077961

申请日：2013-11-12

Applicant: Monsanto Technology, LLC

Inventor： Jennifer R. Hicks ,  Brenda A. Lowe ,  Brian J. Martinell ,  John Phillips ,  John P. Tamulonis ,  Edward J. Williams

IPC: C12N15/82 ,  A01H5/00

CPC classification number: C12N15/8289 ,  A01H1/04 ,  A01H5/00 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8283 ,  C12N15/8286

Abstract: The invention is directed, in an embodiment, to a method for producing a transgenic plant comprising: providing a substantially homozygous plant line suitable for transformation; selecting a subline of the plant line having reduced heterogeneity; transforming plant materials from the subline with a transgenic construct that confers a desired trait to at least one transformed plant; recovering at least one transgenic event from the transformation step; and selecting a transgenic event exhibiting a desirable level of the desired trait using plants of the subline as control. The invention is also directed to a method for producing a plant having a desired trait. The invention also provides transgenic plants produced according to these methods.

Abstract translation: 本发明在一个实施方案中涉及生产转基因植物的方法，其包括：提供适于转化的基本上纯合的植物系; 选择具有减小的异质性的植物系的一个子系; 用具有将所需性状赋予至少一种转化植物的转基因构建体从该亚系转化植物材料; 从转化步骤回收至少一个转基因事件; 并且使用该子系的植物作为对照选择表现出期望性状的期望水平的转基因事件。 本发明还涉及一种用于生产具有所需性状的植物的方法。 本发明还提供根据这些方法生产的转基因植物。