公开(公告)号：US20220251588A1

公开(公告)日：2022-08-11

申请号：US17630465

申请日：2020-08-04

Applicant: Monsanto Technology LLC

Inventor： Charles Lester Armstrong ,  Michelle Lee Gasper ,  Andrei Y. Kouranov ,  Richard J. Lawrence ,  Samuel Sukhwan Yang

IPC: C12N15/82 ,  C12Q1/6876

Abstract: Methods and compositions for evaluating the efficiency of chromosomal rearrangement are provided. In some examples, systems comprising a first DNA molecule comprising the N-terminal portion of a first split reporter coding sequence linked to the C-terminal portion of a second split reporter coding sequence via a first intron, and a second DNA molecule comprising the N-terminal portion of said second split reporter coding sequence linked to the C-terminal portion of said first split reporter coding sequence via a second intron. The introns comprise at least one target site recognized by a genome editing reagent, such as a recombinase or endonuclease, such that recombination results in expression of the first or second reporter coding sequence following splicing of the introns.