公开(公告)号：US20240209385A1

公开(公告)日：2024-06-27

申请号：US18596328

申请日：2024-03-05

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Andrei Y. Kouranov ,  Rosemarie Kuehn ,  Richard J. Lawrence ,  Ervin D. Nagy ,  Linda Rymarquis ,  Veena Veena

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8216

Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for us for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.

公开(公告)号：US11732268B2

公开(公告)日：2023-08-22

申请号：US16311749

申请日：2017-06-27

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Elysia K. Krieger ,  Richard J. Lawrence ,  Zarir E. Vaghchhipawala ,  Jianping Xu ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8202 ,  C12N15/8205 ,  C12N15/8213

Abstract: The present disclosure is in the field of plant transformation. The disclosure provides methods for increasing the rate of site-directed integration of a sequence of interest in plants. The disclosure also provides methods for increasing the efficiency of Rhizobiales-mediated plant transformation.

公开(公告)号：US20140283166A1

公开(公告)日：2014-09-18

申请号：US14209731

申请日：2014-03-13

Applicant: Monsanto Technology, LLC

Inventor： Paul S. Chomet ,  Jonathan C. Lamb ,  Richard J. Lawrence

IPC: A01H1/02

CPC classification number: A01H1/02 ,  C12N15/8213

Abstract: The current invention provides methods for creating a block of genetically linked transgenic traits or a megalocus that can be transmitted as a single genetic unit. The present invention further provides methods for trait introgression to other plants, varieties or species using the megaloci of the invention. Also provided are plants, seeds, and plant parts comprising the megaloci.

Abstract translation: 本发明提供了用于产生遗传连锁转基因性状的块或可作为单个遗传单位传播的巨晶的方法。 本发明还提供了使用本发明的巨球藻对其它植物，品种或物种进行性状渗入的方法。 还提供了包含巨藻的植物，种子和植物部分。

公开(公告)号：US20220135991A1

公开(公告)日：2022-05-05

申请号：US17503235

申请日：2021-10-15

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Andrei Y. Kouranov ,  Rosemarie Kuehn ,  Richard J. Lawrence ,  Ervin D. Nagy ,  Linda Rymarquis ,  Veena Veena

IPC: C12N15/82

Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.

公开(公告)号：US20210321586A1

公开(公告)日：2021-10-21

申请号：US17308978

申请日：2021-05-05

Applicant: Monsanto Technology LLC

Inventor： Paul S. Chomet ,  Jonathan C. Lamb ,  Richard J. Lawrence

IPC: A01H1/02

Abstract: The current invention provides methods for creating a block of genetically linked transgenic traits or a megalocus that can be transmitted as a single genetic unit. The present invention further provides methods for trait introgression to other plants, varieties or species using the megaloci of the invention. Also provided are plants, seeds, and plant parts comprising the megaloci.

公开(公告)号：US11952578B2

公开(公告)日：2024-04-09

申请号：US18146434

申请日：2022-12-26

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Andrei Y. Kouranov ,  Rosemarie Kuehn ,  Richard J. Lawrence ,  Ervin D. Nagy ,  Linda Rymarquis ,  Veena Veena

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8216

Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.

公开(公告)号：US11566254B2

公开(公告)日：2023-01-31

申请号：US17503235

申请日：2021-10-15

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Andrei Y. Kouranov ,  Rosemarie Kuehn ,  Richard J. Lawrence ,  Ervin D. Nagy ,  Linda Rymarquis ,  Veena Veena

IPC: C12N15/82

Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.

公开(公告)号：US20220348943A1

公开(公告)日：2022-11-03

申请号：US17748857

申请日：2022-05-19

Applicant: Monsanto Technology LLC

Inventor： Charles L. Armstrong ,  Edward J. Cargill ,  Fenggao Dong ,  Jonathan C. Lamb ,  Huachun W. Larue ,  Richard J. Lawrence ,  Thomas S. Ream

IPC: C12N15/82 ,  A01H1/08 ,  C12N9/12 ,  C12N9/22 ,  C12N15/10

Abstract: Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.

公开(公告)号：US20220251588A1

公开(公告)日：2022-08-11

申请号：US17630465

申请日：2020-08-04

Applicant: Monsanto Technology LLC

Inventor： Charles Lester Armstrong ,  Michelle Lee Gasper ,  Andrei Y. Kouranov ,  Richard J. Lawrence ,  Samuel Sukhwan Yang

IPC: C12N15/82 ,  C12Q1/6876

Abstract: Methods and compositions for evaluating the efficiency of chromosomal rearrangement are provided. In some examples, systems comprising a first DNA molecule comprising the N-terminal portion of a first split reporter coding sequence linked to the C-terminal portion of a second split reporter coding sequence via a first intron, and a second DNA molecule comprising the N-terminal portion of said second split reporter coding sequence linked to the C-terminal portion of said first split reporter coding sequence via a second intron. The introns comprise at least one target site recognized by a genome editing reagent, such as a recombinase or endonuclease, such that recombination results in expression of the first or second reporter coding sequence following splicing of the introns.

公开(公告)号：US11186843B2

公开(公告)日：2021-11-30

申请号：US15120110

申请日：2015-02-27

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Brent Brower-Toland ,  Andrei Y. Kouranov ,  Rosemarie Kuehn ,  Richard J. Lawrence ,  Ervin D. Nagy ,  Linda Rymarquis ,  Veena Veena

IPC: C12N15/81 ,  C12N15/82

Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.