公开(公告)号：US20230255157A1

公开(公告)日：2023-08-17

申请号：US17935438

申请日：2022-09-26

Applicant: Monsanto Technology LLC

Inventor： Christopher T. Bauer ,  David V. Butruille ,  Edward J. Cargill ,  Daniel Challis ,  Fenggao Dong ,  Fengxing Du ,  Brian W. Gardunia ,  Yang Ju Im ,  Jonathan C. Lamb ,  Piyaraj Nan Newton ,  Pierre Sehabiague ,  Ruth A. Wagner ,  Shengqiang Zhong

IPC: A01H1/08 ,  A01H1/04

CPC classification number: A01H1/08 ,  A01H1/04

Abstract: The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.

公开(公告)号：US20210235644A1

公开(公告)日：2021-08-05

申请号：US17049957

申请日：2019-04-25

Applicant: Monsanto Technology LLC

Inventor： Christopher T. Bauer ,  David V. Butruille ,  Edward J. Cargill ,  Daniel Challis ,  Fenggao Dong ,  Fengxing Du ,  Brian W. Gardunia ,  Yang Ju lm ,  Jonathan C. Lamb ,  Piyaraj Nan Newton ,  Pierre Sehabiague ,  Ruth A. Wagner ,  Shengqiang Zhong

IPC: A01H1/08 ,  A01H1/04

Abstract: The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.

公开(公告)号：US11483991B2

公开(公告)日：2022-11-01

申请号：US17049957

申请日：2019-04-25

Applicant: Monsanto Technology LLC

Inventor： Christopher T. Bauer ,  David V. Butruille ,  Edward J. Cargill ,  Daniel Challis ,  Fenggao Dong ,  Fengxing Du ,  Brian W. Gardunia ,  Yang Ju Im ,  Jonathan C. Lamb ,  Piyaraj Nan Newton ,  Pierre Sehabiague ,  Ruth A. Wagner ,  Shengqiang Zhong

IPC: A01H1/08 ,  A01H6/46 ,  A01H1/04

Abstract: The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.