公开(公告)号：US12241074B2

公开(公告)日：2025-03-04

申请号：US16472140

申请日：2017-12-21

Applicant: Monsanto Technology LLC

Inventor： Ty J. Barten ,  Edward J. Cargill ,  Jonathan C. Lamb ,  Bryce Lemke ,  Linda A. Rymarquis ,  Dennis H. Yang

IPC: C12N15/82 ,  A01H1/06 ,  A01H5/10 ,  A01H6/46 ,  C07K14/415

Abstract: Disclosed herein are plants exhibiting a semi-dwarf phenotype with reduced plant height compared to control wildtype plants. Some of the disclosed semi-dwarf plants comprise at least one non-natural brachytic mutation in which the activity of a BR2 gene is reduced. Also disclosed are methods for producing a semi-dwarf corn plant using a CRISPR based genome editing system.

公开(公告)号：US11627736B2

公开(公告)日：2023-04-18

申请号：US16276617

申请日：2019-02-15

Applicant: Monsanto Technology LLC

Inventor： Ty J. Barten ,  David V. Butruille ,  Edward J. Cargill ,  Charles Dietrich ,  Jose R. Gomez ,  Michael A. Hall ,  Manuel Oyervides Garcia

IPC: C12N15/00 ,  A01M7/00 ,  B05B1/20 ,  A01C21/00 ,  A01H3/04

Abstract: Methods for providing compositions to corn fields prior to harvesting are provided herein. These methods provide an extended time period for the use of lower height or standard height farm equipment in-season in corn fields, while reducing the risk of damage to the corn plants. These methods also allow for late season access with lower height or standard height farm equipment, while reducing the risk of damage to the corn plants.

公开(公告)号：US20210235644A1

公开(公告)日：2021-08-05

申请号：US17049957

申请日：2019-04-25

Applicant: Monsanto Technology LLC

Inventor： Christopher T. Bauer ,  David V. Butruille ,  Edward J. Cargill ,  Daniel Challis ,  Fenggao Dong ,  Fengxing Du ,  Brian W. Gardunia ,  Yang Ju lm ,  Jonathan C. Lamb ,  Piyaraj Nan Newton ,  Pierre Sehabiague ,  Ruth A. Wagner ,  Shengqiang Zhong

IPC: A01H1/08 ,  A01H1/04

Abstract: The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.

公开(公告)号：US20220348943A1

公开(公告)日：2022-11-03

申请号：US17748857

申请日：2022-05-19

Applicant: Monsanto Technology LLC

Inventor： Charles L. Armstrong ,  Edward J. Cargill ,  Fenggao Dong ,  Jonathan C. Lamb ,  Huachun W. Larue ,  Richard J. Lawrence ,  Thomas S. Ream

IPC: C12N15/82 ,  A01H1/08 ,  C12N9/12 ,  C12N9/22 ,  C12N15/10

Abstract: Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.

公开(公告)号：US11483991B2

公开(公告)日：2022-11-01

申请号：US17049957

申请日：2019-04-25

Applicant: Monsanto Technology LLC

Inventor： Christopher T. Bauer ,  David V. Butruille ,  Edward J. Cargill ,  Daniel Challis ,  Fenggao Dong ,  Fengxing Du ,  Brian W. Gardunia ,  Yang Ju Im ,  Jonathan C. Lamb ,  Piyaraj Nan Newton ,  Pierre Sehabiague ,  Ruth A. Wagner ,  Shengqiang Zhong

IPC: A01H1/08 ,  A01H6/46 ,  A01H1/04

Abstract: The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.

公开(公告)号：US10881057B2

公开(公告)日：2021-01-05

申请号：US16276615

申请日：2019-02-15

Applicant: Monsanto Technology LLC

Inventor： Paul Cannon ,  Edward J. Cargill ,  Charles T. Foresman ,  Michael A. Hall ,  Scott C. Johnson ,  John A. Miklos

IPC: A01H1/02 ,  C12N15/82

Abstract: Methods for improving the efficiency and productivity of hybrid corn seed production are provided herein. Various methods to improve the transfer of pollen from male corn plants to female corn plants, and thus increase yield, are provided herein. Without being limiting, these methods include varying the height of male and female corn plants in a field, as well as varying the number, arrangement, and ratio of male-to-female rows in a field.

公开(公告)号：US20240110194A1

公开(公告)日：2024-04-04

申请号：US18377421

申请日：2023-10-06

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  David Vincent Butruille ,  Edward J. Cargill ,  Yurong Chen ,  Megan Elizabeth Hassebrock ,  Thomas Ream ,  Jennifer Rinehart ,  Mary Ann Saltarikos ,  Michelle Folta Valentine

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/11

CPC classification number: C12N15/8205 ,  C12N9/22 ,  C12N15/11

Abstract: The present disclosure provides novel compositions and methods for collectively transforming or genetically modifying a population of distinct germplasm of different germplasms or having different genotypes. The compositions of the present disclosure may include a population of distinct germplasm, such as embryo explants, and a heterologous polynucleotide molecule, a ribonucleoprotein, or a site-specific nuclease. The methods of the present disclosure may include one or more steps of explant preparation, explant rehydration, Rhizobiales bacterium inoculation and co-culture or particle bombardment, bud induction, extended bud induction, and/or regeneration or development of genetically modified plants or plant parts. The methods provided herein may include transforming at least one plant cell of the embryo explants with a heterologous polynucleotide. The methods provided herein also include methods of regenerating or growing a plurality of genetically modified plants or plant parts from the population of transformed or edited plant cells or explants and comparing, selecting or screening one or more genotypes having an improved phenotype or other culturing characteristic.

公开(公告)号：US20230323381A1

公开(公告)日：2023-10-12

申请号：US18182207

申请日：2023-03-10

Applicant: Monsanto Technology LLC

Inventor： Paul Cannon ,  Edward J. Cargill ,  Charles T. Foresman ,  Michael A. Hall ,  Scott C. Johnson ,  John A. Miklos

IPC: C12N15/82

CPC classification number: C12N15/8262 ,  C12N15/8297 ,  C12N15/8218

Abstract: Methods for improving the efficiency and productivity of hybrid corn seed production are provided herein. Various methods to improve the transfer of pollen from male corn plants to female corn plants, and thus increase yield, are provided herein. Without being limiting, these methods include varying the height of male and female corn plants in a field, as well as varying the number, arrangement, and ratio of male-to-female rows in a field.

公开(公告)号：US20190169596A1

公开(公告)日：2019-06-06

申请号：US16275200

申请日：2019-02-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Charles L. ARMSTRONG ,  Edward J. Cargill ,  Fenggao Dong ,  Jonathan C. Lamb ,  Huachun W. Larue ,  Richard J. Lawrence ,  Thomas S. Ream

IPC: C12N15/10 ,  C12N9/22 ,  C12N9/12

Abstract: Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.

公开(公告)号：US20230255157A1

公开(公告)日：2023-08-17

申请号：US17935438

申请日：2022-09-26

Applicant: Monsanto Technology LLC

Inventor： Christopher T. Bauer ,  David V. Butruille ,  Edward J. Cargill ,  Daniel Challis ,  Fenggao Dong ,  Fengxing Du ,  Brian W. Gardunia ,  Yang Ju Im ,  Jonathan C. Lamb ,  Piyaraj Nan Newton ,  Pierre Sehabiague ,  Ruth A. Wagner ,  Shengqiang Zhong

IPC: A01H1/08 ,  A01H1/04

CPC classification number: A01H1/08 ,  A01H1/04

Abstract: The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.